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Heart Lysate

WB Whole Tissue Lysate Tissue Lysate
产品编号 ABIN2456425
发货至: 中国
  • 资源
    Rhesus Monkey
    应用范围
    Western Blotting (WB)
    产品特性
    Monkey (Rhesus) heart tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70 °C. The monkey (Rhesus) heart tissue total protein is provided in a buffer including HEPES ( pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the heart tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The heart tissue is then Western analyzed by either GAPDH or beta-actin antibody, and the expression level is consistent with each lot.

    Source: Monkey (Rhesus) heart tissue
    Diagnosis: Normal
    Tissue or Cell type: Heart
    Lysate Type
    Tissue Lysate
    Lysate Fraction
    Whole Tissue Lysate
  • 应用备注
    Heart lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    2 mg/mL
    缓冲液
    HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.
    注意事项
    Repeated freezing and thawing is not recommended.
    储存条件
    4 °C/-80 °C
    储存方法
    Store at 2-8 °C for continuous use. For extended storage, freeze working aliquots at -70 °C.
    有效期
    6 months
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