Duodenum Lysate
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- 资源
- Human
- 应用范围
- Western Blotting (WB)
- 产品特性
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Duodenum tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH 7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10 % glycerol, 1 % NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5 % glycerol, 1 % sodium dodecylsulfate, 0.01 % bromophenol blue) containing 5 % beta-mercaptoethanol.
Source: Human duodenum tissue
Diagnosis: Normal
Tissue or Cell type: Duodenum - Lysate Type
- Tissue Lysate
- Lysate Fraction
- Whole Tissue Lysate
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BIRC3 293T Cell Transient Overexpression Lysate(Denatured)
BIRC3 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
APLP2 293T Cell Transient Overexpression Lysate(Denatured)APLP2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
BAG1 293T Cell Transient Overexpression Lysate(Denatured)BAG1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
VNN1 293T Cell Transient Overexpression Lysate(Denatured)VNN1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
CYP26A1 293T Cell Transient Overexpression Lysate(Denatured)CYP26A1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
ACTB 293T Cell Transient Overexpression Lysate(Denatured)ACTB WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
ACTG2 293T Cell Transient Overexpression Lysate(Denatured)ACTG2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
ACTN4 293T Cell Transient Overexpression Lysate(Denatured)ACTN4 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
ACTN1 293T Cell Transient Overexpression Lysate(Denatured)ACTN1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
ACVR1B 293T Cell Transient Overexpression Lysate(Denatured)ACVR1B WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 2 mg/mL
- 缓冲液
- SDS sample buffer containing 5 % beta-mercaptoethanol.
- 注意事项
- Repeated freezing and thawing is not recommended.
- 储存条件
- 4 °C/-80 °C
- 储存方法
- Store at 2-8 °C for continuous use. For extended storage, freeze working aliquots at -70 °C.
- 有效期
- 6 months
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