EVL HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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- 抗原 See all EVL products
- EVL (Ena-Vasodilator Stimulated Phosphoprotein (EVL))
- 宿主
- 人
- 资源
- 人细胞
- 应用范围
- Immunoprecipitation (IP), Western Blotting (WB)
- 原理
- Transient Overexpression Lysate
- 特异性
- Non-denatured
- 产品特性
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EVL HEK293 Cell Transient Overexpression Lysate (Non-Denatured)
Used Plasmid: pCMV-EVL full length - Lysed Cells
- HEK 293T Cells
- Lysate Type
- Overexpression Lysate
- Lysate Fraction
- Whole Cell Lysate
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- 应用备注
- Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
- 说明
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Product Quality tested by: Transient overexpression cell lysate was tested with Anti-EVL antibody by Western Blots.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 2 mg/mL
- 缓冲液
- Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
- 注意事项
- Aliquot to avoid repeated freezing and thawing.
- 储存条件
- -80 °C
- 储存方法
- Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
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