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AF5Q31 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

AFF4 WB, IP Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
产品编号 ABIN1339583
发货至: 中国
  • 抗原 See all AFF4 products
    AFF4 (AF4/FMR2 Family, Member 4 (AFF4))
    宿主
    资源
    人细胞
    应用范围
    Western Blotting (WB), Immunoprecipitation (IP)
    原理
    Transient Overexpression Lysate
    特异性
    Non-denatured
    产品特性
    AF5Q31 HEK293 Cell Transient Overexpression Lysate (Non-Denatured)
    Used Plasmid: pCMV-AF5Q31 full length
    Lysed Cells
    HEK 293T Cells
    Lysate Type
    Overexpression Lysate
    Lysate Fraction
    Whole Cell Lysate
  • 应用备注
    Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
    说明

    Product Quality tested by: Transient overexpression cell lysate was tested with Anti-AF5Q31 antibody by Western Blots.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    2 mg/mL
    缓冲液
    Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
    注意事项
    Aliquot to avoid repeated freezing and thawing.
    储存条件
    -80 °C
    储存方法
    Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
  • 抗原
    AFF4 (AF4/FMR2 Family, Member 4 (AFF4))
    别名
    AFF4 (AFF4 产品)
    别名
    AFF4 Lysate, RA_m002_jsmFBA6Br Lysate, AF5Q31 Lysate, MCEF Lysate, Alf4 Lysate, Laf4l Lysate, AF4/FMR2 family member 4 L homeolog Lysate, AF4/FMR2 family member 4 Lysate, AF4/FMR2 family, member 4 Lysate, aff4.L Lysate, AFF4 Lysate, aff4 Lysate, Aff4 Lysate
    背景
    Full Gene Name: AF4/FMR2 family, member 4
    Synonyms: AF5Q31,MCEF,MGC75036
    基因ID
    27125
    NCBI登录号
    NP_055238, NM_014423
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