TNF alpha ELISA 试剂盒
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- 抗原 See all TNF alpha ELISA试剂盒
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
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适用
- 小鼠
- 应用范围
- ELISA
- 产品特性
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ELISA kit for the detection of TNF alpha in the research laboratory
Alternative Names: Mouse TNF alpha ELISA kit, TNF alpha ELISA kit, TNFalpha ELISA kit, TNFa ELISA kit, TNFa ELISA kit, Tumor Necrosis Factor ELISA kit, TNFSF2 ELISA kit, Cachectin ELISA kit, Differentiation-inducing factor ELISA kit, DIF ELISA kit, Necrosin ELISA kit, Cytotoxin ELISA kit - Top Product
- Discover our top product TNF alpha ELISA Kit
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Tumor Necrosis Factor alpha (TNF alpha) ELISA Kit
TNF alpha 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha 适用: 人 AA 77-233 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha 适用: 兔 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha 适用: 大鼠 Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha 适用: 小鼠 AA 80-235 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha 适用: 大鼠 AA 80-235 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha 适用: 人 Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Optimal conditions to be determined by end user
- 实验流程
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This mouse TNFalpha Enzyme Immunometric Assay (EIA) kit is a complete kit for the determintation of mouse TNFalpha in biological fluids. Please read the complete kit insert before performing this assay. The kit uses a monoclonal antibody to mouse TNFalpha immobilized on a microtiter plate to bind the mouse TNFalpha in the standards or sample. A recombinant mouse TNFalpha Standard is provided in the kit. After a short incubation the excess sample or standard is washed out and a polyclonal antibody to mouse TNFalpha is added. This antibody binds to the mouse TNFalpha captured on the plate. After a short incubation the excess antibody is washed out and donkey antirabbitIgG conjugated to Horseradish peroxidase is added, which binds to the polyclonal mouse TNFalpha antibody. Excess conjugate iswashed out and substrate is added.After a short incubation, the enzyme reaction is stopped and the color generated is read at 450 nm. The measured optical density is directly proportional to the concentration of mouse TNFalpha in either standards or samples.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 储存方法
- Store at 2-8 °C.
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- 抗原 See all TNF alpha ELISA试剂盒
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
- 别名
- TNF alpha (TNF alpha 产品)
- 背景
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Tumor Necrosis Factoralpha (TNF alpha) is a 17.5 kDa, 157 amino acid protein that is a potent lymphoid factor, which exerts cytotoxic effects on a wide range of tumor cells and other target cells. TNF alpha has been suggested to play a proinflammatory role and has been detected in synovial fluid of patients with rheumatoid arthritis. It is the primary mediator of immune regulation. The biosynthesis of TNF alpha is tightly controlled, being produced in extremely small quantities in quiescent cells, but is a major secreted factor in activated cells.
Synonyms: Mouse TNF alpha ELISA kit, TNF alpha ELISA kit, TNFalpha ELISA kit, TNFa ELISA kit, TNFa ELISA kit, Tumor Necrosis Factor ELISA kit, TNFSF2 ELISA kit, Cachectin ELISA kit, Differentiation-inducing factor ELISA kit, DIF ELISA kit, Necrosin ELISA kit, Cytotoxin ELISA kit. - 途径
- NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
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