IGF1 ELISA 试剂盒
Quick Overview for IGF1 ELISA 试剂盒 (ABIN930359)
抗原
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实验类型
应用范围
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产品特性
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ELISA kit for the detection of Human IGF1 in the research laboratory
Alternative Names: IGF1 ELISA kit, Insulin-like Growth Factor 1 ELISA kit, Human IGF1 ELISA kit, IGF-1 ELISA kit -
纯化方法
- Immunoaffinity chromatography
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Insulin-Like Growth Factor 1 (IGF1) ELISA Kit
IGF1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor 1 (IGF1) ELISA KitIGF1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor 1 (IGF1) ELISA KitIGF1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor 1 (IGF1) ELISA KitIGF1 适用: 小鸡 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor 1 (IGF1) ELISA KitIGF1 适用: 犬 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor 1 (IGF1) ELISA KitIGF1 适用: Pig Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor 1 (IGF1) ELISA KitIGF1 适用: 大鼠 AA 49-118 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Insulin-Like Growth Factor 1 (IGF1) ELISA KitIGF1 适用: Cow Colorimetric Competition ELISA 12.5 ng/mL - 800 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor 1 (IGF1) ELISA KitIGF1 适用: 鸭 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor 1 (IGF1) ELISA KitHigh Sensitivity IGF1 适用: 人 Colorimetric Sandwich ELISA 0.19 ng/mL - 12 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal conditions to be determined by end user
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实验流程
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This ELISA for IGFI E20 is a socalled SandwichAssay using two specific and high affinity antibodies. The IGFI in the samples binds to the first antibody coated on the microtiterplate, the second specific antiIGFIantibody binds inturn to the immobilised IGFI. The second antibody is biotinylated, the subsequently incubated StreptavidinperoxidaseEnzyme Conjugate will bind to it, and thus in the final substrate incubation step colour development will be catalysed quantitatively depending on the IGFIlevel of the samples. The Standards of the ELISA E20 are preparedfrom recombinant IGFI in concentrations of 1, 5, 15, 30 and 50 ng/mL.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store at 2-8 °C.
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- IGF1 (Insulin-Like Growth Factor 1 (IGF1))
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别名
- IGF1
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背景
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Insulin-like growth factors (IGF) I and II play a pivotal role in regulating the proliferation, differentiation and specific functions of many cell types. IGFI is identical with Somatomedin C (SmC) and has a molecular weight of 7649 Da. Its major regulators are growth hormone (GH) and nutrition, although its production in specific tissues is affected by a multitude of tropic hormones and other peptide growth factors. In contrast to many other peptide hormones, IGFs are avidly bound to specific binding proteins (IGFBP). The seven classes of IGFBPs which are known at present either bind IGFI and IGFII with similar affinities or show a preference for IGFII.
Synonyms: IGF1 ELISA kit, Insulin-like Growth Factor 1 ELISA kit. -
途径
- RTK signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation
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