IGF1 ELISA 试剂盒 (Insulin-Like Growth Factor 1)

Details for Product IGF1 ELISA Kit No. ABIN411285, 供应商: Log in to see
抗原
  • IGF-I
  • IGF1A
  • IGFI
  • C730016P09Rik
  • Igf-1
  • Igf-I
  • Npt2B
  • IGF-1
  • IGFIA
  • IGF-IB
  • igf1
  • IGF-1L
  • IGF-1a
  • IGF1
  • igf-1
  • igf1-A
  • igf1.S
  • xigf1
  • insulin like growth factor 1
  • insulin-like growth factor 1
  • insulin-like growth factor I
  • insulin like growth factor 1 L homeolog
  • IGF1
  • Igf1
  • LOC100136741
  • igf1
  • LOC678666
  • igf1.L
抗原表位
AA 49-118
适用
大鼠
其他选择
Kits with alternative reactivity to:
40
35
31
18
16
14
13
12
6
6
6
5
4
3
1
1
1
1
1
1
实验类型
Sandwich ELISA
检测范围
62.5-4000 pg/mL
最低检测浓度
62.5 pg/mL
应用范围
ELISA
选项
Supplier
Log in to see
Supplier Product No.
Log in to see
原理 Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat IGF-1
品牌 PicoKine™
样品类型 Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Analytical Method Quantitative
检测方法 Colorimetric
免疫原 Expression system for standard: E.coli
Immunogen sequence: G49-A118
特异性 Expression system for standard: E.coli
Immunogen sequence: G49-A118
交叉反应 (详细) There is detectable cross-reactivity with IGF-2< 1 % .
预测反应 Hamster
灵敏度 <5pg/mL
试剂未包括 Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Plasmids, Primers & others Plasmids, Primers & others IGF1 products on genomics-online (e.g. as negative or positive controls)
别名 IGF1 (IGF1 ELISA Kit 摘要)
背景

Protein Function: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation (By similarity). .

Background: Insulin-like growth factor 1(IGF-1) that was once called somatomedin C, is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. Human IGF1 is a single chain 70-amino acid polypeptide cross-linked by 3 disulfide bridges, with a calculated molecular mass of 7.6 kD. The IGF1 gene, mapped on 12q22-q24.1, contains 5 exons. Exons 1-4 encode the 195-amino acid precursor(IGF1B), and exons 1, 2, 3, and 5 encode the 153-residue peptide(IGF1A). The structure of IGF1 resembles that of IGF2. And the IGF1 and IGF2 genes have complex structures with multiple promoters. The expression of both genes is regulated at the levels of transcription, RNA processing, and translation. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. Moreover, approximately 98 % of IGF-1 is always bound to one of 6 binding proteins(IGF-BP). Furthermore, IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death.

Synonyms: Insulin-like growth factor I,IGF-I,Somatomedin,Igf1,Igf-1,

Full Gene Name: Insulin-like growth factor I

Cellular Localisation: Secreted.
基因ID 24482
UniProt P08025
途径 RTK signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation
应用备注 Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
说明

Sequence similarities: Belongs to the insulin family.

板类型 Pre-coated
实验流程 rat IGF-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGF-1 has been precoated onto 96-well plates. Standards(E.coli, G49-A118) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGF-1is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IGF-1 amount of sample captured in plate.
实验流程

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL rat IGF-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat IGF-1 standard solution and each sample be measured in duplicate.

实验精密度
  • Sample 1: n=16, Mean(pg/ml): 527, Standard deviation: 32.67, CV(%): 6.2
  • Sample 2: n=16, Mean(pg/ml): 1441, Standard deviation: 56.20, CV(%): 3.9
  • Sample 3: n=16, Mean(pg/ml): 2641, Standard deviation: 140, CV(%): 5.3,
  • Sample 1: n=24, Mean(pg/ml): 497, Standard deviation: 41.25, CV(%): 8.3
  • Sample 2: n=24, Mean(pg/ml): 1532, Standard deviation: 82.73, CV(%): 5.4
  • Sample 3: n=24, Mean(pg/ml): 2737, Standard deviation: 156, CV(%): 5.7
限制 仅限研究用
注意事项 Avoid multiple freeze-thaw cycles.
储存条件 -20 °C,4 °C
储存方法 Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
有效期 12 months
厂商提供的图像
ELISA image for Insulin-Like Growth Factor 1 (IGF1) ELISA Kit (ABIN411285) Rat IGF-1 PicoKine ELISA Kit standard curve
有引用在: Ozdemir, Aksu, Baykara, Ates, Sisman, Kiray, Buyuk, Uysal: "Effects of administration of subtoxic doses of acetaminophen on liver and blood levels of insulin-like growth factor-1 in rats." in: Toxicology and industrial health, Vol. 32, Issue 1, pp. 39-46, 2016 (PubMed).

Wang, Wang, Liang, Liu, Shi, Bai, Lin, Magaye, Zhao: "Expression and clinical significance of IGF-1, IGFBP-3, and IGFBP-7 in serum and lung cancer tissues from patients with non-small cell lung cancer." in: OncoTargets and therapy, Vol. 6, pp. 1437-44, 2013 (PubMed).

Cai, Li, Wang, Liu, Yang, Chen, Yin, Tan, Zhu, Pan, Wang, Lu: "Apoptosis of bone marrow mesenchymal stem cells caused by homocysteine via activating JNK signal." in: PLoS ONE, Vol. 8, Issue 5, pp. e63561, 2013 (PubMed).

Aksu, Baykara, Kiray, Gurpinar, Sisman, Ekerbicer, Tas, Gokdemir-Yazar, Uysal: "Serum IGF-1 levels correlate negatively to liver damage in diabetic rats." in: Biotechnic & histochemistry : official publication of the Biological Stain Commission, Vol. 88, Issue 3-4, pp. 194-201, 2013 (PubMed).

Ni, Sun, Fu, Wang, Guo, Tian, Wei: "IGF-1 promotes the development and cytotoxic activity of human NK cells." in: Nature communications, Vol. 4, pp. 1479, 2013 (PubMed).

Baykara, Aksu, Buyuk, Kiray, Sisman, Baykara, Dayi, Tas, Ozdemir, Arda, Uysal et al.: "Progesterone treatment decreases traumatic brain injury induced anxiety and is correlated with increased serum IGF-1 levels; prefrontal cortex, amygdala, hippocampus neuron density; and reduced serum ..." in: Biotechnic & histochemistry : official publication of the Biological Stain Commission, Vol. 88, Issue 5, pp. 250-7, 2013 (PubMed).

背景 Ozdemir, Baykara, Aksu, Kiray, Sisman, Cetin, Dayi, Gurpinar, Uysal, Arda: "Relationship between circulating IGF-1 levels and traumatic brain injury-induced hippocampal damage and cognitive dysfunction in immature rats." in: Neuroscience letters, Vol. 507, Issue 1, pp. 84-9, 2012 (PubMed).

Sussenbach, Steenbergh, Holthuizen: "Structure and expression of the human insulin-like growth factor genes." in: Growth regulation, Vol. 2, Issue 1, pp. 1-9, 1993 (PubMed).

Rotwein, Pollock, Didier, Krivi: "Organization and sequence of the human insulin-like growth factor I gene. Alternative RNA processing produces two insulin-like growth factor I precursor peptides." in: The Journal of biological chemistry, Vol. 261, Issue 11, pp. 4828-32, 1986 (PubMed).

Rinderknecht, Humbel: "The amino acid sequence of human insulin-like growth factor I and its structural homology with proinsulin." in: The Journal of biological chemistry, Vol. 253, Issue 8, pp. 2769-76, 1978 (PubMed).

您还需要查找其他产品吗?