MERTK ELISA 试剂盒

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.

Tissue Specificity: Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. Highly expressed in testis, ovary, prostate, lung, and kidney, with lower expression in spleen, small intestine, colon, and liver.

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Mer standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue lysates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Mer standard solution and each sample be measured in duplicate.

• Sample 1: n=16, Mean(pg/ml): 1124, Standard deviation: 58.45, CV(%): 5.2
• Sample 2: n=16, Mean(pg/ml): 3287, Standard deviation: 210.4, CV(%): 6.4
• Sample 3: n=16, Mean(pg/ml): 5853, Standard deviation: 280.9, CV(%): 4.8,
• Sample 1: n=24, Mean(pg/ml): 1374, Standard deviation: 92.1, CV(%): 6.7
• Sample 2: n=24, Mean(pg/ml): 3467, Standard deviation: 249.6, CV(%): 7.2
• Sample 3: n=24, Mean(pg/ml): 6124, Standard deviation: 306.2, CV(%): 5

Protein Function: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll- like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. .

Background: Proto-oncogene tyrosine-protein kinase MER is an enzyme that in humans is encoded by the MERTK gene. This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type(immunoglobulin-like) domains, and one tyrosine kinase domain. Its gene is mapped to chromosome 2q14.1. Mer encodes a 984-amino acid protein with a calculated molecular mass of 109 kD. It is expressed in numerous neoplastic B- and T-cell lines. Mutations in this gene have been associated with disruption of the retinal pigment epithelium(RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa(RP)

Synonyms: Tyrosine-protein kinase Mer,2.7.10.1,Proto-oncogene c-Mer,Receptor tyrosine kinase MerTK,MERTK,MER,

Full Gene Name: Tyrosine-protein kinase Mer