MERTK ELISA 试剂盒

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.

Tissue Specificity: Expressed predominantly in the hematopoietic lineages: macrophages, NK cells, NKT cells, dendritic cells and platelets. .

Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL mouse MER standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, cell lysates, tissue homogenates or serum to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse MER standard solution and each sample is measured in duplicate.

• Sample 1: n=16, Mean(pg/ml): 568, Standard deviation: 25.56, CV(%): 4.5
• Sample 2: n=16, Mean(pg/ml): 1965, Standard deviation: 102.18, CV(%): 5.2
• Sample 3: n=16, Mean(pg/ml): 3223, Standard deviation: 183.7, CV(%): 5.7,
• Sample 1: n=24, Mean(pg/ml): 790, Standard deviation: 45.82, CV(%): 5.8
• Sample 2: n=24, Mean(pg/ml): 2135, Standard deviation: 140.91, CV(%): 6.6
• Sample 3: n=24, Mean(pg/ml): 3632, Standard deviation: 257.9, CV(%): 7.1

Protein Function: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll- like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. .

Background: Proto-oncogene tyrosine-protein kinase MER, also called MERTK is an enzyme that in humans is encoded by the MERTK gene. This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type(immunoglobulin-like) domains, and one tyrosine kinase domain. This gene is mapped to 2q13. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium(RPE) as a regulator of rod outer segments fragments phagocytosis. This gene also plays an important role in inhibition of Toll-like receptors(TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.

Synonyms: Tyrosine-protein kinase Mer,2.7.10.1,Proto-oncogene c-Mer,Receptor tyrosine kinase MerTK,Mertk,Mer,

Full Gene Name: Tyrosine-protein kinase Mer