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MERTK ELISA 试剂盒

MERTK 适用: 人 Colorimetric Sandwich ELISA 0.15-40 ng/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN2703325
发货至: 中国
  • 抗原 See all MERTK ELISA试剂盒
    MERTK (C-Mer Proto-Oncogene Tyrosine Kinase (MERTK))
    适用
    • 5
    • 3
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.15-40 ng/mL
    最低检测浓度
    0.15 ng/mL
    应用范围
    ELISA
    原理
    Human Mer ELISA Kit for cell culture supernatants, plasma, and serum samples.
    样品类型
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    特异性
    Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: ANGPTL3, beta IG-H3, CA9, Cathepsin B, CD23, CHI3L1, CTLA-4 (CD152), DKK-4, DPPIV (CD26), EDA-A2, Erythropoietin R, FGF-6, FGF-9, Gas 1, GITR (TNFRSF18), IGFBP-5, IL-1 F5 (FIL1 delta), IL-1 F6 (FIL1 epsilon), IL-1 F7 (FIL1 zeta), IL-1 F8 (FIL1 eta), IL-1 F9 (IL-1 H1), IL-1 F10 (IL-1HY2), IL-1 R5, IL-21 (IL-17C), IL-18, IL-20, IL-34, IL-5 R alpha, IL-10 R alpha, Layilin, Leptin R, Marapsin, MMP-7, P-Cadherin, Prostasin, PSMA (FOLH1), SIGIRR, TGF beta R3, Coagulation Factor III (Tissue Factor, F3), TWEAK (TNFSF12)
    灵敏度
    0.15 ng/mL
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 应用备注
    Recommended Dilution for serum and plasma samples2 fold
    样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • Qin, Wang, Ma, Yang, Fu, Liang, Huang, Yang, Zhong: "The association of Tyro3/Axl/Mer signaling with inflammatory response, disease activity in patients with primary Sjögren's syndrome." in: Joint, bone, spine : revue du rhumatisme, Vol. 82, Issue 4, pp. 258-63, (2015) (PubMed).

    Breunung: "[Possibilities and limitations of an early prognosis in rearing premature infants]." in: Monatsschrift für Kinderheilkunde, Vol. 113, Issue 10, pp. 577-80 (PubMed).

  • 抗原 See all MERTK ELISA试剂盒
    MERTK (C-Mer Proto-Oncogene Tyrosine Kinase (MERTK))
    别名
    Mer (MERTK 产品)
    别名
    MER ELISA Kit, RP38 ELISA Kit, c-mer ELISA Kit, Eyk ELISA Kit, Mer ELISA Kit, Nyk ELISA Kit, nmf12 ELISA Kit, rdy ELISA Kit, C-EYK ELISA Kit, MERTK ELISA Kit, DKFZp469D2215 ELISA Kit, si:ch1073-268l12.2 ELISA Kit, MER proto-oncogene, tyrosine kinase ELISA Kit, c-mer proto-oncogene tyrosine kinase ELISA Kit, c-mer proto-oncogene tyrosine kinase a ELISA Kit, MERTK ELISA Kit, Mertk ELISA Kit, mertka ELISA Kit
    背景
    Gene Names: MERTK MER
    Protein names: Tyrosine-protein kinase Mer (EC 2.7.10.1) (Proto-oncogene c-Mer) (Receptor tyrosine kinase MerTK)
    基因ID
    10461
    UniProt
    Q12866
    途径
    RTK signaling
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