PD-1 ELISA 试剂盒
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Quick Overview for PD-1 ELISA 试剂盒 (ABIN8070496)
抗原
See all PD-1 (PDCD1) ELISA试剂盒抗原表位
适用
检测方法
实验类型
应用范围
样品类型
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原理
- Phosphotyrosine PD-1 ELISA Kit is a convenient and sensitive assay to detect Phosphotyrosine PD-1 in human cell lysate samples.
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Analytical Method
- Quantitative
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特异性
- This ELISA antibody pair detects human Phospho PD-1.
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组件
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- Pre-coated 96-well microplate
- Positive Control
- Anti-phospho Detection Antibody
- HRP-Streptavidin
- Wash Buffer
- Assay Diluent
- Lysis Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Protease and Phosphatase inhibitors
- Shaker
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for ELISA data analysis
- Tubes to prepare the positive control or sample dilutions
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Programmed Cell Death 1 (PDCD1) ELISA Kit
PDCD1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 适用: Various Species Colorimetric Competition ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 适用: 小鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 适用: 人 AA 25-167 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma (heparin), Serum
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 适用: Various Species Colorimetric Competition ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 适用: 人 Colorimetric Sandwich ELISA 0.156-10 ng/mL Plasma, Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and positive control as instructed.
- Add 100 μL of positive control and sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
- Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL prepared HRP-Streptavidin to each well. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
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- PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))
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别名
- PD1
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背景
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Gene Symbols: PDCD1, PD1
Protein Name (Synonyms): Programmed cell death protein 1 (Protein PD-1, hPD-1)
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基因ID
- 5133
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UniProt
- Q15116
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途径
- Cancer Immune Checkpoints
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