IKK alpha ELISA 试剂盒
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Quick Overview for IKK alpha ELISA 试剂盒 (ABIN8056626)
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See all IKK alpha (CHUK) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
质量等级
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最低检测浓度
- 3.12 pg/mL
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原理
- High Sensitive ELISA Kit for Conserved Helix Loop Helix Ubiquitous Kinase (CHUK)
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Analytical Method
- Quantitative
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特异性
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This assay has high sensitivity and excellent specificity for detection of High Sensitive Conserved Helix Loop Helix Ubiquitous Kinase (CHUK).
No significant cross-reactivity or interference between High Sensitive Conserved Helix Loop Helix Ubiquitous Kinase (CHUK) and analogues was observed. -
灵敏度
- < 1.11 pg/mL
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组件
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- Pre-coated, 96-well strip plate
- Plate sealer
- Standard
- Standard Diluent
- Detection Reagent A
- Assay Diluent A
- Detection Reagent B
- Assay Diluent B
- TMB Substrate
- Stop Solution
- Wash Buffer (30X)
- Instruction manual
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试剂未包括
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- Microplate reader with 450 ± 10nm filter.
- Precision single or multi-channel pipettes and disposable tips.
- Eppendorf Tubes for diluting samples.
- Deionized or distilled water.
- Absorbent paper for blotting the microtiter plate.
- Container for Wash Solution
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conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) ELISA Kit
CHUK 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) ELISA KitHigh Sensitivity CHUK 适用: 人 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Lysate, Tissue Homogenate
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样本量
- 100 μL
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实验时间
- 3 h
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板类型
- Pre-coated
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实验流程
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1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Conserved Helix Loop Helix Ubiquitous Kinase (CHUK) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Conserved Helix Loop Helix Ubiquitous Kinase (CHUK) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
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限制
- 仅限研究用
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注意事项
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. -
储存条件
- 4 °C,-20 °C
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储存方法
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- For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
- For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit. - For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.
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有效期
- 6 months
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- IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))
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别名
- Conserved Helix Loop Helix Ubiquitous Kinase
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背景
- TCF16, IKK1, IKK-alpha, IkBKA, NFKBIKA, IKKA, Inhibitor of nuclear factor kappa-B kinase subunit alpha, Transcription factor 16, I-kappa-B kinase 1
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UniProt
- O15111
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途径
- PI3K-Akt Signaling, NF-kappaB Signaling, RTK signaling, TCR Signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins
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