Insulin ELISA 试剂盒
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北京 101111
Quick Overview for Insulin ELISA 试剂盒 (ABIN8055892)
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See all Insulin (INS) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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原理
- Sandwich Human C-Peptide ELISA Kit to quantitate Human C-Peptide in cell culture supernatants, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: human peptide
Capture antibody and Detection antibody: polyclonal antibody from goat, biotinylated polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Insulin (INS) ELISA Kit
INS 适用: 大鼠 Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin (INS) ELISA KitINS 适用: 人 Colorimetric Competition ELISA 61.7 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Insulin (INS) ELISA KitINS 适用: 小鼠 Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin (INS) ELISA KitINS 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 nlU/mL - 1000 nlU/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin (INS) ELISA KitINS 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 nlU/mL - 1000 nlU/mL Cell Culture Supernatant, Plasma, Serum
Insulin (INS) ELISA KitINS 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Insulin (INS) ELISA KitINS 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Insulin (INS) ELISA KitINS 适用: Cow Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin (INS) ELISA KitINS 适用: Pig Colorimetric Competition ELISA 61.7 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin (INS) ELISA KitINS 适用: 猴 Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 2.3%, 5.6%, 2.9%
Inter-Assay Precision (CV%): 5.1%, 3.8%, 4.4% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- Insulin (INS)
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别名
- INS
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背景
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Background: C-Peptide, or connecting peptide, is a short peptide chain that is produced during the synthesis of insulin. When proinsulin is cleaved to form insulin, C-Peptide is released as a byproduct. It serves as a useful biomarker for assessing insulin production in the body because its levels reflect the amount of insulin secreted by the pancreas. Unlike insulin, C-Peptide has a longer half-life in the bloodstream, making it easier to measure. Elevated levels of C-Peptide can indicate insulinoma or insulin resistance, while low levels may suggest type 1 diabetes or pancreatic dysfunction. Understanding C-Peptide levels can help in diagnosing and managing various metabolic disorders.
Gene Full Name: insulin
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基因ID
- 3630
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UniProt
- P01308
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途径
- NF-kappaB Signaling, RTK signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
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