IKK alpha ELISA 试剂盒
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Quick Overview for IKK alpha ELISA 试剂盒 (ABIN7830598)
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See all IKK alpha (CHUK) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- CHUK/IKBKB (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CHUK/IKBKB expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠, 大鼠
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conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) ELISA Kit
CHUK 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) ELISA KitHigh Sensitivity CHUK 适用: 人 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Lysate, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))
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别名
- CHUK
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背景
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Synonyms: IKBKA|IKK-alpha|IKK1|IKKA|NFKBIKA|TCF16
Gene Description: conserved helix-loop-helix ubiquitous kinase
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基因ID
- 1147
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UniProt
- O15111
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途径
- PI3K-Akt Signaling, NF-kappaB Signaling, RTK signaling, TCR Signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins
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