BRAF ELISA 试剂盒
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Quick Overview for BRAF ELISA 试剂盒 (ABIN7830449)
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See all BRAF ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- BRAF (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of BRAF expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠, 大鼠
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B-Raf proto-oncogene, serine/threonine kinase (BRAF) ELISA Kit
BRAF 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
B-Raf proto-oncogene, serine/threonine kinase (BRAF) ELISA KitBRAF 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))
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别名
- BRAF
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背景
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Synonyms: B-RAF1|BRAF1|FLJ95109|MGC126806|MGC138284|RAFB1
Gene Description: v-raf murine sarcoma viral oncogene homolog B1
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基因ID
- 673
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UniProt
- P15056
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途径
- MAPK Pathway, RTK signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy
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