PTP4A2 ELISA 试剂盒
PTP4A2
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all PTP4A2 products
- PTP4A2 (Protein tyrosine Phosphatase Type IVA, Member 2 (PTP4A2))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human PTP4A2 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 有效期
- 6 months
-
- 抗原 See all PTP4A2 products
- PTP4A2 (Protein tyrosine Phosphatase Type IVA, Member 2 (PTP4A2))
- 别名
- PTP4A2 (PTP4A2 产品)
- 别名
- wu:fc05f09 ELISA Kit, wu:fi84b06 ELISA Kit, zgc:101724 ELISA Kit, MGC53390 ELISA Kit, MGC80084 ELISA Kit, MGC132077 ELISA Kit, PTP4A2 ELISA Kit, ptp4a2 ELISA Kit, Prl-2 ELISA Kit, HH13 ELISA Kit, HH7-2 ELISA Kit, HU-PP-1 ELISA Kit, OV-1 ELISA Kit, PRL-2 ELISA Kit, PRL2 ELISA Kit, PTP4A ELISA Kit, PTPCAAX2 ELISA Kit, ptp-IV1a ELISA Kit, ptp-IV1b ELISA Kit, protein tyrosine phosphatase type IVA, member 2b ELISA Kit, protein tyrosine phosphatase 4a2 ELISA Kit, protein tyrosine phosphatase type IVA, member 2 ELISA Kit, protein tyrosine phosphatase type IVA, member 2 L homeolog ELISA Kit, ptp4a2b ELISA Kit, ptp4a2 ELISA Kit, PTP4A2 ELISA Kit, ptp4a2.L ELISA Kit, Ptp4a2 ELISA Kit
- 基因ID
- 8073
- UniProt
- Q12974
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