GNB2L1 ELISA 试剂盒
GNB2L1
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all GNB2L1 products
- GNB2L1 (Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 2-Like 1 (GNB2L1))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human GNB2L1/RACK1 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
-
- 抗原 See all GNB2L1 products
- GNB2L1 (Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 2-Like 1 (GNB2L1))
- 别名
- GNB2L1 (RACK1 (GNB2L1 产品)
- 别名
- MGC53289 ELISA Kit, GNB2L1 ELISA Kit, AL033335 ELISA Kit, GB-like ELISA Kit, Gnb2-rs1 ELISA Kit, Rack1 ELISA Kit, p205 ELISA Kit, RACK1 ELISA Kit, H12.3 ELISA Kit, HLC-7 ELISA Kit, PIG21 ELISA Kit, rack1 ELISA Kit, wu:fb80d08 ELISA Kit, wu:fk65d12 ELISA Kit, gnb2-rs1 ELISA Kit, h12.3 ELISA Kit, hlc-7 ELISA Kit, pig21 ELISA Kit, C12-3 ELISA Kit, c12.3 ELISA Kit, guanine nucleotide binding protein, beta 2, related sequence 1 ELISA Kit, receptor for activated C kinase 1 ELISA Kit, guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1 ELISA Kit, receptor for activated C kinase 1 L homeolog ELISA Kit, Guanine nucleotide-binding protein subunit beta-2-like 1 ELISA Kit, gnb2l1 ELISA Kit, RACK1 ELISA Kit, Rack1 ELISA Kit, rack1.L ELISA Kit, rack-1 ELISA Kit
- 基因ID
- 10399
- UniProt
- P63244
- 途径
- cAMP Metabolic Process, Positive Regulation of Endopeptidase Activity
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