CDC42 ELISA 试剂盒
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Quick Overview for CDC42 ELISA 试剂盒 (ABIN6951508)
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See all CDC42 ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- Human CDC42 ELISA Kit.
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Analytical Method
- Quantitative
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42) ELISA Kit
CDC42 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42) ELISA KitCDC42 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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有效期
- 6 months
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- CDC42 (Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42))
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别名
- CDC42
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基因ID
- 998
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UniProt
- P60953
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途径
- MAPK Pathway, Microtubule Dynamics, RTK signaling, WNT signaling, TCR Signaling, EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Maintenance of Protein Location, Skeletal Muscle Fiber Development, Signaling Events mediated by VEGFR1 and VEGFR2, EGFR Downregulation, VEGF Signaling
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