ATP6V1F ELISA 试剂盒
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- 抗原 See all ATP6V1F products
- ATP6V1F (ATPase, H+ Transporting, Lysosomal 14kDa, V1 Subunit F (ATP6V1F))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human ATP6V1F ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
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- 抗原 See all ATP6V1F products
- ATP6V1F (ATPase, H+ Transporting, Lysosomal 14kDa, V1 Subunit F (ATP6V1F))
- 别名
- ATP6V1F (ATP6V1F 产品)
- 别名
- Atp6s14 ELISA Kit, Vma7 ELISA Kit, atp6s14 ELISA Kit, atp6v1f ELISA Kit, vatf ELISA Kit, ATP6S14 ELISA Kit, VATF ELISA Kit, 1110004G16Rik ELISA Kit, ATPase H+ transporting V1 subunit F ELISA Kit, ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F S homeolog ELISA Kit, ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F L homeolog ELISA Kit, ATPase, H+ transporting, lysosomal V1 subunit F ELISA Kit, Atp6v1f ELISA Kit, atp6v1f.S ELISA Kit, ATP6V1F ELISA Kit, atp6v1f.L ELISA Kit
- 基因ID
- 9296
- UniProt
- Q16864
- 途径
- Transition Metal Ion Homeostasis, Proton Transport
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