COL4A3BP ELISA 试剂盒
COL4A3BP
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all COL4A3BP ELISA试剂盒
- COL4A3BP (Collagen, Type IV, alpha 3 (Goodpasture Antigen) Binding Protein (COL4A3BP))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human COL4A3BP ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our best selling COL4A3BP ELISA Kit
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Collagen, Type IV, alpha 3 (Goodpasture Antigen) Binding Protein (COL4A3BP) ELISA Kit
COL4A3BP 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
-
- 抗原 See all COL4A3BP ELISA试剂盒
- COL4A3BP (Collagen, Type IV, alpha 3 (Goodpasture Antigen) Binding Protein (COL4A3BP))
- 别名
- COL4A3BP (COL4A3BP 产品)
- 别名
- COL4A3BP ELISA Kit, 2810404O15Rik ELISA Kit, 9230101K08Rik ELISA Kit, AU016711 ELISA Kit, CERT ELISA Kit, GPBP ELISA Kit, col4a3bp ELISA Kit, wu:fc37f11 ELISA Kit, zgc:92147 ELISA Kit, CERTL ELISA Kit, STARD11 ELISA Kit, cert ELISA Kit, certl ELISA Kit, cetp ELISA Kit, gpbp ELISA Kit, stard11 ELISA Kit, collagen type IV alpha 3 binding protein ELISA Kit, collagen, type IV, alpha 3 (Goodpasture antigen) binding protein ELISA Kit, collagen, type IV, alpha 3 (Goodpasture antigen) binding protein a ELISA Kit, collagen, type IV, alpha 3 (Goodpasture antigen) binding protein L homeolog ELISA Kit, COL4A3BP ELISA Kit, Col4a3bp ELISA Kit, col4a3bpa ELISA Kit, col4a3bp.L ELISA Kit
- 基因ID
- 10087
- UniProt
- Q9Y5P4
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