COL9A1 ELISA 试剂盒
-
- 抗原 See all COL9A1 ELISA试剂盒
- COL9A1 (Collagen, Type IX, alpha 1 (COL9A1))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human COL9A1 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product COL9A1 ELISA Kit
-
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 有效期
- 6 months
-
- 抗原 See all COL9A1 ELISA试剂盒
- COL9A1 (Collagen, Type IX, alpha 1 (COL9A1))
- 别名
- COL9A1 (COL9A1 产品)
- 别名
- cb339 ELISA Kit, sb:cb339 ELISA Kit, zgc:85892 ELISA Kit, wu:fa12a12 ELISA Kit, wu:fa12d02 ELISA Kit, wu:fc22c08 ELISA Kit, wu:fc38d08 ELISA Kit, edm6 ELISA Kit, MGC82441 ELISA Kit, DJ149L1.1.2 ELISA Kit, EDM6 ELISA Kit, MED ELISA Kit, STL4 ELISA Kit, Col9a-1 ELISA Kit, collagen, type IX, alpha 1b ELISA Kit, collagen, type IX, alpha 1 L homeolog ELISA Kit, collagen type IX alpha 1 chain ELISA Kit, collagen, type IX, alpha 1 ELISA Kit, collagen alpha-1(IX) chain ELISA Kit, col9a1b ELISA Kit, col9a1.L ELISA Kit, COL9A1 ELISA Kit, Col9a1 ELISA Kit, LOC101102992 ELISA Kit
- 基因ID
- 1297
- UniProt
- P20849
-