DcTrailR1 /2 ELISA 试剂盒
-
- 抗原 See all DcTrailR1 /2 (TNFRSF10A/B) products
- DcTrailR1 /2 (TNFRSF10A/B) (Tumor Necrosis Factor Receptor Superfamily, Member 23 (TNFRSF10A/B))
- 适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse DcTRAIL R1/TNFRSF23 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse DcTRAIL R1.
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 有效期
- 6 months
-
- 抗原 See all DcTrailR1 /2 (TNFRSF10A/B) products
- DcTrailR1 /2 (TNFRSF10A/B) (Tumor Necrosis Factor Receptor Superfamily, Member 23 (TNFRSF10A/B))
- 别名
- DcTRAIL R1 / TNFRSF23 (TNFRSF10A/B 产品)
- 别名
- Dctrailr1 ELISA Kit, Tnfrh1 ELISA Kit, Tnfrsf1al1 ELISA Kit, mDcTrailr1 ELISA Kit, mSOB ELISA Kit, tumor necrosis factor receptor superfamily, member 23 ELISA Kit, Tnfrsf23 ELISA Kit
- UniProt
- Q9ER63
-