HIST2H2BE ELISA 试剂盒
HIST2H2BE
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
- 抗原 See all HIST2H2BE ELISA试剂盒
- HIST2H2BE (Histone H2B Type 2-E (HIST2H2BE))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human HIST2H2BE ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human HIST2H2BE.
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling HIST2H2BE ELISA Kit
- Top Product
- Discover our top product HIST2H2BE ELISA Kit
-
Histone H2B Type 2-E (HIST2H2BE) ELISA Kit
HIST2H2BE 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all HIST2H2BE ELISA试剂盒
- HIST2H2BE (Histone H2B Type 2-E (HIST2H2BE))
- 别名
- HIST2H2BE (HIST2H2BE 产品)
- 别名
- RGD1562346 ELISA Kit, AV127319 ELISA Kit, H2b-613 ELISA Kit, T25626 ELISA Kit, DKFZp469C0528 ELISA Kit, H2b ELISA Kit, GL105 ELISA Kit, H2B ELISA Kit, H2B.1 ELISA Kit, H2BFQ ELISA Kit, H2BGL105 ELISA Kit, H2BQ ELISA Kit, histone cluster 2 H2B family member E ELISA Kit, histone cluster 2, H2be ELISA Kit, histone cluster 1, H2bl ELISA Kit, histone cluster 2 H2B family member e ELISA Kit, histone H2B ELISA Kit, Hist2h2be ELISA Kit, HIST2H2BE ELISA Kit, Hist1h2bl ELISA Kit, H2B ELISA Kit
- UniProt
- Q16778
-
You are here:
