PDGFRB ELISA 试剂盒
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- 抗原 See all PDGFRB ELISA试剂盒
- PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse PDGF R beta ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse PDGF R beta.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our best selling PDGFRB ELISA Kit
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- Discover our top product PDGFRB ELISA Kit
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Platelet Derived Growth Factor Receptor beta (PDGFRB) ELISA Kit
PDGFRB 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Platelet Derived Growth Factor Receptor beta (PDGFRB) ELISA KitPDGFRB 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Platelet Derived Growth Factor Receptor beta (PDGFRB) ELISA KitPDGFRB 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Platelet Derived Growth Factor Receptor beta (PDGFRB) ELISA KitPDGFRB 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Platelet Derived Growth Factor Receptor beta (PDGFRB) ELISA KitPDGFRB 适用: 小鼠 Colorimetric Sandwich ELISA 23.44 pg/mL - 1500 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Platelet Derived Growth Factor Receptor beta (PDGFRB) ELISA KitPDGFRB 适用: 人 Colorimetric Sandwich ELISA 25 pg/mL-18000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all PDGFRB ELISA试剂盒
- PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))
- 别名
- PDGF R beta (PDGFRB 产品)
- 别名
- AI528809 ELISA Kit, CD140b ELISA Kit, PDGFR-1 ELISA Kit, Pdgfr ELISA Kit, CD140B ELISA Kit, IBGC4 ELISA Kit, JTK12 ELISA Kit, PDGFR ELISA Kit, PDGFR1 ELISA Kit, platelet derived growth factor receptor, beta polypeptide ELISA Kit, platelet derived growth factor receptor beta ELISA Kit, Pdgfrb ELISA Kit, PDGFRB ELISA Kit
- UniProt
- P05622
- 途径
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
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