JNK ELISA 试剂盒
This 小鼠 JNK ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 JNK.
Quick Overview for JNK ELISA 试剂盒 (ABIN6730536)
抗原
See all JNK (MAPK8) ELISA试剂盒
JNK (MAPK8)
(Mitogen-Activated Protein Kinase 8 (MAPK8))
适用
小鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
样品类型
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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原理
- Mouse JNK1 ELISA Kit.
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Analytical Method
- Quantitative
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特异性
- This ELISA antibody pair recognizes Mouse JNK1.
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Mitogen-Activated Protein Kinase 8 (MAPK8) ELISA Kit
MAPK8 适用: 人, 小鼠, 大鼠 pThr183 Colorimetric Cell ELISA Cell Culture Cells
Mitogen-Activated Protein Kinase 8 (MAPK8) ELISA KitMAPK8 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 6 months
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- JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))
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别名
- JNK1
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UniProt
- A3KBF5
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途径
- MAPK Pathway, WNT signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins
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