MEK1 ELISA 试剂盒
-
- 抗原 See all MEK1 (MAP2K1) ELISA试剂盒
- MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))
-
适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse MAP2K1 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse MAP2K1.
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product MAP2K1 ELISA Kit
-
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) ELISA Kit
MAP2K1 适用: 人, 大鼠, 小鼠 pSer217 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) ELISA KitMAP2K1 适用: 人, 大鼠, 小鼠 pSer217 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) ELISA KitMAP2K1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) ELISA KitMAP2K1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) ELISA KitMAP2K1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all MEK1 (MAP2K1) ELISA试剂盒
- MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))
- 别名
- MAP2K1 (MAP2K1 产品)
- 别名
- Mek1 ELISA Kit, CFC3 ELISA Kit, MAPKK1 ELISA Kit, MEK1 ELISA Kit, MKK1 ELISA Kit, PRKMK1 ELISA Kit, MEKK1 ELISA Kit, Prkmk1 ELISA Kit, mek-2 ELISA Kit, si:ch211-242m18.2 ELISA Kit, wu:fj56a12 ELISA Kit, wu:fj61b01 ELISA Kit, zgc:56557 ELISA Kit, ATMEK1 ELISA Kit, F20B18.180 ELISA Kit, F20B18_180 ELISA Kit, MAP kinase/ ERK kinase 1 ELISA Kit, MITOGEN ACTIVATED PROTEIN KINASE KINASE ELISA Kit, MITOGEN ACTIVATED PROTEIN KINASE KINASE 1 ELISA Kit, NMAPKK ELISA Kit, MEK ELISA Kit, mitogen activated protein kinase kinase 1 ELISA Kit, mitogen-activated protein kinase kinase 1 ELISA Kit, mitogen-activated protein kinase kinase 1 L homeolog ELISA Kit, MAP kinase/ ERK kinase 1 ELISA Kit, Map2k1 ELISA Kit, MAP2K1 ELISA Kit, map2k1.L ELISA Kit, map2k1 ELISA Kit, MEK1 ELISA Kit
- UniProt
- P31938
- 途径
- MAPK Pathway, RTK signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling
-
