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TGFBR1 ELISA 试剂盒
TGFBR1
适用: 小鼠
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
TGFBR1ELISA Kit详情
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TGFBR1
(Transforming Growth Factor, beta Receptor 1 (TGFBR1))
适用
All reactivities for TGFBR1 ELISA试剂盒
小鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
原理
Mouse TGF-beta RI/ALK-5 ELISA Kit.
样品类型
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
特异性
This ELISA antibody pair recognizes Mouse TGF-beta RI.
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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使用细节
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应用备注
Optimal working dilution should be determined by the investigator.
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
TGFBR1目标详情
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TGFBR1
(Transforming Growth Factor, beta Receptor 1 (TGFBR1))
别名
TGF-beta RI (ALK-5 (TGFBR1 产品 )
别名
AAT5 ELISA Kit, ACVRLK4 ELISA Kit, ALK-5 ELISA Kit, ALK5 ELISA Kit, LDS1A ELISA Kit, LDS2A ELISA Kit, MSSE ELISA Kit, SKR4 ELISA Kit, TGFR-1 ELISA Kit, aat5 ELISA Kit, acvrlk4 ELISA Kit, alk-5 ELISA Kit, alk5 ELISA Kit, lds1a ELISA Kit, lds2a ELISA Kit, skr4 ELISA Kit, tgfr-1 ELISA Kit, TGFBR1 ELISA Kit, x-trr1 ELISA Kit, AU017191 ELISA Kit, Alk-5 ELISA Kit, TbetaR-I ELISA Kit, TbetaRI ELISA Kit, Alk5 ELISA Kit, Skr4 ELISA Kit, Tgfr-1 ELISA Kit, tbetaR-I ELISA Kit, tgfbr1 ELISA Kit, zgc:123263 ELISA Kit, transforming growth factor beta receptor 1 ELISA Kit, transforming growth factor beta receptor I ELISA Kit, transforming growth factor beta receptor I S homeolog ELISA Kit, transforming growth factor, beta receptor I ELISA Kit, transforming growth factor, beta receptor 1 ELISA Kit, transforming growth factor, beta receptor 1 a ELISA Kit, TGFBR1 ELISA Kit, tgfbr1 ELISA Kit, tgfbr1.S ELISA Kit, Tgfbr1 ELISA Kit, tgfbr1a ELISA Kit
UniProt
Q64729
途径
Growth Factor Binding
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