COL18A1 ELISA 试剂盒
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- 抗原 See all COL18A1 ELISA试剂盒
- COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156 pg/mL - 10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich ELISA for Quantitative Detection of Antigen
- 样品类型
- Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- 产品特性
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Synonyms: Alpha 1 collagen type 18 (XVIII)(COL18A1), Alpha 1 type XVIII collagen, Antiangiogenic agent, COL18A1, Collagen alpha 1(XVIII) chain, Collagen type XVIII alpha 1, FLJ27325, FLJ34914, KNO, KNO1, MGC74745, Multi functional protein MFP, OTTMUSP00000023764, OTTMUSP00000023766, Procollagen type XVIII alpha 1
Background: Endostatin is a naturally-occurring 20- kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And it is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.
Gene Name: COL18A1
Production: Natural and recombinant human Endostatin. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: E.coli, Immunogen sequence: H1154-S1419
- Top Product
- Discover our top product COL18A1 ELISA Kit
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Collagen, Type XVIII, alpha 1 (COL18A1) ELISA Kit
COL18A1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Collagen, Type XVIII, alpha 1 (COL18A1) ELISA KitCOL18A1 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Collagen, Type XVIII, alpha 1 (COL18A1) ELISA KitCOL18A1 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Collagen, Type XVIII, alpha 1 (COL18A1) ELISA KitCOL18A1 适用: 大鼠 Colorimetric Competition ELISA 1.23 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Collagen, Type XVIII, alpha 1 (COL18A1) ELISA KitCOL18A1 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Collagen, Type XVIII, alpha 1 (COL18A1) ELISA KitWide Range COL18A1 适用: 人 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Collagen, Type XVIII, alpha 1 (COL18A1) ELISA KitCOL18A1 适用: 人 Colorimetric Competition ELISA 4.94 ng/mL - 400 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Collagen, Type XVIII, alpha 1 (COL18A1) ELISA KitCOL18A1 适用: 人 AA 1154-1335 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Serum
Collagen, Type XVIII, alpha 1 (COL18A1) ELISA KitCOL18A1 适用: 小鼠 AA 1591-1774 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Collagen, Type XVIII, alpha 1 (COL18A1) ELISA KitCOL18A1 适用: 大鼠 AA 1591-1774 Colorimetric Sandwich ELISA
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- 应用备注
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Endostatin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or human milk to each empty well. It is recommended that each human Endostatin standard solution and each sample be measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 156pg/mL-10000pg/mL
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
-
- 抗原 See all COL18A1 ELISA试剂盒
- COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))
- 别名
- Endostatin (COL18A1 产品)
- 别名
- KNO ELISA Kit, KNO1 ELISA Kit, KS ELISA Kit, cb371 ELISA Kit, cb529 ELISA Kit, fc96d06 ELISA Kit, sb:cb529 ELISA Kit, wu:fb47h10 ELISA Kit, wu:fb77g12 ELISA Kit, wu:fc50f04 ELISA Kit, wu:fc96d06 ELISA Kit, wu:fk02h12 ELISA Kit, kno ELISA Kit, kno1 ELISA Kit, collagen type XVIII alpha 1 chain ELISA Kit, collagen type XVIII alpha 1 chain a ELISA Kit, collagen, type XVIII, alpha 1 ELISA Kit, collagen type XVIII alpha 1 ELISA Kit, COL18A1 ELISA Kit, col18a1a ELISA Kit, Col18a1 ELISA Kit, col18a1 ELISA Kit
- 物质类
- Chemical
- 基因ID
- 80781
- NCBI登录号
- NP_085059
- UniProt
- P39060
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