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- 适用
- 小鼠, 兔
- 宿主
- 山羊
- 应用范围
- BioImaging (BI), Immunofluorescence (IF), Immunostaining (ISt), Multiplex Assay (MA), Standard (STD), Western Blotting (WB)
- 原理
- This kit is suitable for fluorescent western blotting, multiplex analysis, including multicolor imaging, utilizing various commercial gel imaging systems.
- 产品特性
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Synonyms: Fluorescent western blotting, Multiplex western blotting, multi-color western blotting, fluorescent labelled antibodies, fluorescent imaging, DyLights, dyLight conjugates
Background: The The DyLight™ multiplex 649/488 Duo fluorescent Western blotting kit is suited for simultaneous detection and quantification of specific protein populations in a biological sample. Using a combination of two antibodies selected for minimal cross reactivity, fluorescent detection method enables simultaneous quantitative analysis of multiple proteins within the same sample on the same blot. The DyLight™ multiplex 649/488 Duo Western blot kit contain all the necessary components that are optimized for the simultaneous detection of multiple proteins on the same blot using DyLight™-dye labeled secondary antibodies that are visualized in different fluorescence channels (649/488). The kit also includes blocking buffer, wash buffer, pre-stained protein standard and an incubation box for convinience and ultimate performance with minimal or no optimization. The fluorescent dyes such as DyLights™ when conjugated to secondary antibodies, offer a variety of benefits over traditional detection methods such as colorimetric and chemiluminescent detection. Multiplex detection using the correct lighting and filter conditions, enables the quantitation of multiple proteins and eliminates the need to strip and reprobe. Other benefits of fluorescent Western blotting include increased sensitivity, excellent signal stability over time as well as precise quantitative analysis with broader dynamic range and high linearity. Due to their exceptional photostability, DyLight™ dye conjugates can be archived and visualized several times without a decrease in signal.
- 组件
- DyLight™ Multiplex 649/488 Duo Western Blot Kit contains Goat anti-mouse IgG (H+L) DyLight™ 488, Goat anti-rabbit IgG (H+L) DyLight™ 649, opal pre-stained protein standard, wash buffer (10x TTBS), blocking buffer (2x) and incubation box.
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Tumor Necrosis Factor alpha (TNF alpha) ELISA Kit
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Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Lipopolysaccharides (LPS) ELISA Kit适用: Various Species Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha 适用: 人 AA 77-233 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 6 (IL6) ELISA KitIL6 适用: 人 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 适用: 人 Colorimetric Sandwich ELISA 1.563-100 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 实验流程
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Production: The DyLight conjugated secondary antibodies were prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit or Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit or Mouse IgG and Rabbit or Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rat and Sheep Serum Proteins. These antibodies will react with heavy chains of rabbit or mouse IgG and with light chains of most rabbit or mouse immunoglobulins. Blocking buffer is specifically formulated to achieve superior reproducible western blotting images using this system. Wash buffer (10X TTBS) was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
- 限制
- 仅限研究用
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- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- The secondary antibodies should be stored at 4°C prior to reconstitution. For extended storage aliquot contents and freeze at -20°C. Avoid freeze/thaw cycles. The wash buffer and blocking buffer can be stored at 2-8°C prior to opening. The pre-stained protein Western standards should be stored at 2-8°C. The pre-stained protein standards can also be stored at room temperature for up to 6 months or at -20°C for up to two years.
- 有效期
- 12 months
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