CITED2 IQ-ELISA 试剂盒
CITED2
适用: 人
qPCR
Sandwich ELISA
Cell Culture Supernatant, Plasma, Serum
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- 抗原 See all CITED2 IQ-ELISA Kits
- CITED2 (Cbp/p300-Interacting Transactivator, with Glu/Asp-Rich Carboxy-terminal Domain, 2 (CITED2))
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适用
- 人
- 检测方法
- qPCR
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Immunoquantitative (PCR-Based) Cited?2 ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Semi-Quantitative
- 产品特性
- IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells, the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.
- 组件
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- Cited?2 Microplate (Item A): 96 well PCR plate coated with anti-Human Cited?2
- Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
- Standards (Item C): 2 vials of recombinant Human Cited?2
- Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
- Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
- Detection Affinity Reagent for Cited?2 (Item F): 2 vials of a 4x concentrated solution of anti-Human Cited?2 affinity reagent
- IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
- Primer Solution (Item I): 1.7 ml vial
- PCR Master Mix (Item J): 1.2 ml vial
- PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
- Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer
- 试剂未包括
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- Real-time PCR instrument, Bio-Rad recommended
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for data analysis
- Tubes to prepare standard or sample dilutions
- Heating block or water bath capable of 80°C
- Top Product
- Discover our top product CITED2 ELISA Kit
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- 应用备注
- The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.
- 说明
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The Immuno-Quantitative ELISA (IQELISA™) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.
- 样本量
- 25 µL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed
- Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C
- Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
- Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
- Add 15μL Primer solution 10μL of PCR master mix to each well
- Run real-time PCR
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
- 有效期
- 6 months
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- 抗原 See all CITED2 IQ-ELISA Kits
- CITED2 (Cbp/p300-Interacting Transactivator, with Glu/Asp-Rich Carboxy-terminal Domain, 2 (CITED2))
- 别名
- Cited-2 (CITED2 产品)
- 别名
- mrg1 IQ-ELISA Kit, p35srj IQ-ELISA Kit, zgc:103418 IQ-ELISA Kit, CITED2 IQ-ELISA Kit, cite2 IQ-ELISA Kit, ASD8 IQ-ELISA Kit, MRG-1 IQ-ELISA Kit, MRG1 IQ-ELISA Kit, P35SRJ IQ-ELISA Kit, VSD2 IQ-ELISA Kit, AI835299 IQ-ELISA Kit, ER154-like IQ-ELISA Kit, Mrg1 IQ-ELISA Kit, Msg2 IQ-ELISA Kit, Cbp/p300-interacting transactivator, with Glu/Asp rich carboxy-terminal domain, 2 IQ-ELISA Kit, Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2 IQ-ELISA Kit, Cbp/p300 interacting transactivator with Glu/Asp rich carboxy-terminal domain 2 IQ-ELISA Kit, Cbp/p300-interacting transactivator 2 IQ-ELISA Kit, Cbp/p300-interacting transactivator, with Glu/Asp rich carboxy-terminal domain, 2 L homeolog IQ-ELISA Kit, cited2 IQ-ELISA Kit, CITED2 IQ-ELISA Kit, cite2 IQ-ELISA Kit, Cited2 IQ-ELISA Kit, cited2.L IQ-ELISA Kit
- 基因ID
- 10370
- UniProt
- Q99967
- 途径
- Tube Formation
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