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IL1A ELISA 试剂盒

IL1A 适用: 人 Colorimetric Sandwich ELISA 0.5-300 pg/mL Cell Lysate, Tissue Lysate
产品编号 ABIN625018
发货至: 中国
  • 抗原 See all IL1A ELISA试剂盒
    IL1A (Interleukin 1 alpha (IL1A))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.5-300 pg/mL
    最低检测浓度
    0.5 pg/mL
    应用范围
    ELISA
    原理
    Human IL-1 alpha (IL-1 F1) ELISA Kit for cell and tissue lysate samples.
    样品类型
    Tissue Lysate, Cell Lysate
    Analytical Method
    Quantitative
    特异性
    The antibody pair provided in this kit recognizes human IL-1 alpha.
    灵敏度
    0.5 pg/mL
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    • Cell lysate buffer
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  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all IL1A ELISA试剂盒
    IL1A (Interleukin 1 alpha (IL1A))
    别名
    IL-1 alpha (IL1A 产品)
    别名
    IL-1A ELISA Kit, IL1 ELISA Kit, IL1-ALPHA ELISA Kit, IL1F1 ELISA Kit, IL-6 ELISA Kit, Il-1a ELISA Kit, IL-1 alpha ELISA Kit, IL-1alpha ELISA Kit, L1A ELISA Kit, interleukin 1 alpha ELISA Kit, interleukin 6 ELISA Kit, IL1A ELISA Kit, IL6 ELISA Kit, Il1a ELISA Kit
    背景
    Gene Names: IL1A IL1F1
    Protein names: Interleukin-1 alpha (IL-1 alpha) (Hematopoietin-1)
    基因ID
    3552
    UniProt
    P01583
    途径
    NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints
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