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FASL ELISA 试剂盒

This Pig FASL ELISA Kit is a Colorimetric ELISA Kit designed to quantify Pig FASL.
产品编号 ABIN5690989
发货至: 中国

Quick Overview for FASL ELISA 试剂盒 (ABIN5690989)

抗原

See all FASL ELISA试剂盒
FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

适用

  • 12
  • 7
  • 6
  • 4
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Pig

检测方法

Colorimetric

实验类型

Sandwich ELISA

应用范围

ELISA

样品类型

Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
  • 原理

    Porcine (pig) Fas Ligand/TNFSF6 ELISA Kit.

    Analytical Method

    Quantitative

    特异性

    This ELISA antibody pair recognizes Porcine (pig) Fas Ligand.

    产品特性

    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    组件

    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate

    试剂未包括

    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • 板类型

    Pre-coated

    实验流程

    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.

    限制

    仅限研究用
  • 储存条件

    4 °C

    有效期

    6 months
  • 抗原 See all FASL ELISA试剂盒

    FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

    别名

    Fas Ligand (TNFSF6

    基因ID

    396726

    UniProt

    Q9BEA8

    途径

    Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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