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FASL ELISA 试剂盒

Name: FASL ELISA 试剂盒
SKU: ABIN1672726
Availability: OutOfStock

小鼠 FASL ELISA Kit, Colorimetric is an assay for quantification of 小鼠 FASL, that has been mentioned in 6+ publications.

ELISA

1 image

(6 references)

产品编号 ABIN1672726

发货至: 中国

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Quick Overview for FASL ELISA 试剂盒 (ABIN1672726)

抗原

See all FASL ELISA试剂盒

FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

抗原表位

AA 132-279

适用

小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

31.2 pg/mL - 2000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Discover our top product FASL ELISA Kit

最低检测浓度

31.2 pg/mL

原理

Sandwich Mouse FASL ELISA Kit to quantitate Mouse Faslg in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Analytical Method

Quantitative

特异性

Expression system for standard: NS0
Immunogen sequence: P132-L279
Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

< 10 pg/mL

组件
1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers
试剂未包括

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Fas Ligand (TNF Superfamily, Member 6) (FASL) ELISA Kit
FASL 适用: 人 AA 134-281 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum

Fas Ligand (TNF Superfamily, Member 6) (FASL) ELISA Kit
FASL 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate

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FASL 适用: Pig Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate

Fas Ligand (TNF Superfamily, Member 6) (FASL) ELISA Kit
FASL 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate

Fas Ligand (TNF Superfamily, Member 6) (FASL) ELISA Kit
FASL 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate

Fas Ligand (TNF Superfamily, Member 6) (FASL) ELISA Kit
FASL 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate

Fas Ligand (TNF Superfamily, Member 6) (FASL) ELISA Kit
FASL 适用: 大鼠 AA 127-278 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum

Fas Ligand (TNF Superfamily, Member 6) (FASL) ELISA Kit
FASL 适用: 人 Colorimetric Sandwich ELISA 31.25-2000 pg/mL Cell Culture Supernatant, Plasma, Serum

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

样本量

100 μL

板类型

Pre-coated

实验流程

ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

实验精密度

Intra-Assay Precision (CV%): 4.4%, 6.6%, 6.4%
Inter-Assay Precision (CV%): 5.4%, 8%, 6.6%

限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

有效期

12 months
Soni, Kaminski, Gangaraju, Adebiyi: "Cisplatin-induced oxidative stress stimulates renal Fas ligand shedding." in: Renal failure, Vol. 40, Issue 1, pp. 314-322, (2018) (PubMed).

Aziz, Cui, Das, Brown, Ardell, Febbraio, Pluskota, Han, Wu, Ballantyne, Smith, Cathcart, Yakubenko: "The Upregulation of Integrin αDβ2 (CD11d/CD18) on Inflammatory Macrophages Promotes Macrophage Retention in Vascular Lesions and Development of Atherosclerosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 198, Issue 12, pp. 4855-4867, (2017) (PubMed).

Wu, Hu, Liu, Cao, Xu, Li, Li: "Nature and mechanisms of hepatocyte apoptosis induced by D-galactosamine/lipopolysaccharide challenge in mice." in: International journal of molecular medicine, Vol. 33, Issue 6, pp. 1498-506, (2014) (PubMed).

Xu, Liu, Wu, Gong, Zhou, Qiao: "Proapoptotic effect of metalloproteinase 9 secreted by trophoblasts on endothelial cells." in: The journal of obstetrics and gynaecology research, Vol. 37, Issue 3, pp. 187-94, (2011) (PubMed).

Xiong, Lu, Zhao, Xu, Bao, Lin, Yang: "Therapy with FasL-gene-modified dendritic cells confers a protective microenvironment in murine pregnancy." in: Fertility and sterility, Vol. 93, Issue 8, pp. 2767-9, (2010) (PubMed).

Han, Qiu, Zhang, Kong, Wang, Wang, Li, Duan, Wang, Song, Wang: "Transplantation of sertoli-islet cell aggregates formed by microgravity: prolonged survival in diabetic rats." in: Experimental biology and medicine (Maywood, N.J.), Vol. 234, Issue 5, pp. 595-603, (2009) (PubMed).
抗原 See all FASL ELISA试剂盒

FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

别名

FASLG

背景

Background: FAS Ligand (FASL) is a 40 kDa type II membrane protein belonging to the tumor necrosis factor family, which induces apoptosis by binding to its receptor, Fas. The human FasL gene consists of approximately 8.0 kb and is split into four exons. This gene consists of 281 amino acids with a calculated M (r) of 31,759 and was mapped on chromosome 1q23. It has an identity of 76.9 % at the amino acid sequence level with mouse FasL. The FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis. FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis. This system may also play a role in modulating the genetic susceptibility of mouse strains to develop T-cell lymphoblastic lymphomas.
Gene Full Name: Fas ligand

基因ID

14103

UniProt

P41047

途径

Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity