FASL ELISA 试剂盒
(4 references)-
- 抗原 See all FASL ELISA试剂盒
- FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))
- 抗原表位
- AA 134-281
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6-1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human FASL
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: CHO
Immunogen sequence: P134-L281 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <2pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: CHO
Immunogen sequence: P134-L281 - Featured
- Discover our best selling FASL ELISA Kit
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- Discover our top product FASL ELISA Kit
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Fas Ligand (TNF Superfamily, Member 6) (FASL) ELISA Kit
FASL 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fas Ligand (TNF Superfamily, Member 6) (FASL) ELISA KitFASL 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fas Ligand (TNF Superfamily, Member 6) (FASL) ELISA KitFASL 适用: Pig Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Fas Ligand (TNF Superfamily, Member 6) (FASL) ELISA KitFASL 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Fas Ligand (TNF Superfamily, Member 6) (FASL) ELISA KitFASL 适用: 小鼠 AA 132-279 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Fas Ligand (TNF Superfamily, Member 6) (FASL) ELISA KitFASL 适用: 大鼠 AA 127-278 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Fas Ligand (TNF Superfamily, Member 6) (FASL) ELISA KitFASL 适用: 大鼠 Colorimetric Sandwich ELISA 90-60000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 板类型
- Pre-coated
- 实验流程
- human FASL ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FASL has been precoated onto 96-well plates. Standards(CHO, P134-L281) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FASL is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human FASL amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human FASL standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human FASL standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 108, Standard deviation: 5.62, CV(%): 5.2
- Sample 2: n=16, Mean(pg/ml): 257, Standard deviation: 12.59, CV(%): 4.9
- Sample 3: n=16, Mean(pg/ml): 619, Standard deviation: 36.52, CV(%): 5.9,
- Sample 1: n=24, Mean(pg/ml): 112, Standard deviation: 9.74, CV(%): 8.7
- Sample 2: n=24, Mean(pg/ml): 249, Standard deviation: 22.66, CV(%): 9.1
- Sample 3: n=24, Mean(pg/ml): 623, Standard deviation: 44.86, CV(%): 7.2
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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: "Proapoptotic effect of metalloproteinase 9 secreted by trophoblasts on endothelial cells." in: The journal of obstetrics and gynaecology research, Vol. 37, Issue 3, pp. 187-94, (2011) (PubMed).
: "Therapy with FasL-gene-modified dendritic cells confers a protective microenvironment in murine pregnancy." in: Fertility and sterility, Vol. 93, Issue 8, pp. 2767-9, (2010) (PubMed).
: "Clinical significance of Fas and FasL protein expression in gastric carcinoma and local lymph node tissues." in: World journal of gastroenterology, Vol. 16, Issue 10, pp. 1274-8, (2010) (PubMed).
: "Transplantation of sertoli-islet cell aggregates formed by microgravity: prolonged survival in diabetic rats." in: Experimental biology and medicine (Maywood, N.J.), Vol. 234, Issue 5, pp. 595-603, (2009) (PubMed).
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: "Proapoptotic effect of metalloproteinase 9 secreted by trophoblasts on endothelial cells." in: The journal of obstetrics and gynaecology research, Vol. 37, Issue 3, pp. 187-94, (2011) (PubMed).
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- 抗原 See all FASL ELISA试剂盒
- FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))
- 别名
- FASLG (FASL 产品)
- 别名
- ALPS1B ELISA Kit, APT1LG1 ELISA Kit, APTL ELISA Kit, CD178 ELISA Kit, CD95-L ELISA Kit, CD95L ELISA Kit, FASL ELISA Kit, TNFSF6 ELISA Kit, fasl ELISA Kit, Fas-L ELISA Kit, Faslg ELISA Kit, Tnfsf6 ELISA Kit, gld ELISA Kit, Apt1Lg1 ELISA Kit, Fasl ELISA Kit, FASLG ELISA Kit, zgc:162027 ELISA Kit, Fas ligand ELISA Kit, Fas ligand L homeolog ELISA Kit, Fas ligand (TNF superfamily, member 6) ELISA Kit, FASLG ELISA Kit, faslg.L ELISA Kit, Fasl ELISA Kit, Faslg ELISA Kit, faslg ELISA Kit
- 背景
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Background: Fas ligand(FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor(TNF) family. Its binding with its receptor induces apoptosis. The human FASL gene consists of approximately 8 kb and is split into 4 exons. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Fas ligand or FasL is a homotrimeric type II transmembrane protein. It signals through trimerization of FasR, which spans the membrane of the "target" cell. This trimerization usually leads to apoptosis, or cell death. Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external matrix metalloproteinase MMP-7.
Synonyms: FasL isoform ,Fasl ,
Full Gene Name: Fas ligand
- 基因ID
- 14103
- UniProt
- Q99PH8
- 途径
- Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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