Glucocorticoid Receptor ELISA 试剂盒
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- 抗原 See all Glucocorticoid Receptor (NR3C1) ELISA试剂盒
- Glucocorticoid Receptor (NR3C1) (Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human GR/NR3C1 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human GR.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 6 months
-
- 抗原 See all Glucocorticoid Receptor (NR3C1) ELISA试剂盒
- Glucocorticoid Receptor (NR3C1) (Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1))
- 别名
- GR (NR3C1 (NR3C1 产品)
- 别名
- GR ELISA Kit, gcr ELISA Kit, grl ELISA Kit, xGR ELISA Kit, gccr ELISA Kit, gr ELISA Kit, zgc:113038 ELISA Kit, NR3C1 ELISA Kit, GCL ELISA Kit, LOC100302444 ELISA Kit, Gcr ELISA Kit, Grl ELISA Kit, Grl-1 ELISA Kit, Grl1 ELISA Kit, GRL ELISA Kit, GCCR ELISA Kit, GCR ELISA Kit, NO ELISA Kit, GR-A ELISA Kit, nr3c1 ELISA Kit, nuclear receptor subfamily 3 group C member 1 ELISA Kit, nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) ELISA Kit, nuclear receptor subfamily 3, group C, member 1 ELISA Kit, glucocorticoid receptor ELISA Kit, NR3C1 ELISA Kit, nr3c1 ELISA Kit, LOC100302444 ELISA Kit, Nr3c1 ELISA Kit
- 基因ID
- 2908
- UniProt
- P04150
- 途径
- Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Regulation of Carbohydrate Metabolic Process
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