EDNRB ELISA 试剂盒
-
- 抗原 See all EDNRB ELISA试剂盒
- EDNRB (Endothelin Receptor Type B (EDNRB))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human EDNRB/ET-BR ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human EDNRB.
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling EDNRB ELISA Kit
- Top Product
- Discover our top product EDNRB ELISA Kit
-
-
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存条件
- 4 °C
- 有效期
- 6 months
-
- 抗原 See all EDNRB ELISA试剂盒
- EDNRB (Endothelin Receptor Type B (EDNRB))
- 别名
- EDNRB (ET-BR (EDNRB 产品)
- 别名
- ABCDS ELISA Kit, ET-B ELISA Kit, ET-BR ELISA Kit, ETB ELISA Kit, ETBR ELISA Kit, ETRB ELISA Kit, HSCR ELISA Kit, HSCR2 ELISA Kit, WS4A ELISA Kit, Ednra ELISA Kit, Etb ELISA Kit, ETR-b ELISA Kit, ETb ELISA Kit, Sox10m1 ELISA Kit, EDNRB ELISA Kit, abcds ELISA Kit, etb ELISA Kit, etrb ELISA Kit, hscr ELISA Kit, hscr2 ELISA Kit, endothelin receptor type B ELISA Kit, EDNRB ELISA Kit, Ednrb ELISA Kit, ednrb ELISA Kit
- 基因ID
- 1910
- UniProt
- P24530
- 途径
- Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process
-