Caspase 9 ELISA 试剂盒
Quick Overview for Caspase 9 ELISA 试剂盒 (ABIN5690838)
抗原
See all Caspase 9 (CASP9) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
-
-
原理
- Human Caspase-9 ELISA Kit.
-
Analytical Method
- Quantitative
-
特异性
- This ELISA antibody pair recognizes Human Caspase-9.
-
产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
-
组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
-
试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
-
-
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) ELISA Kit
CASP9 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) ELISA KitCASP9 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) ELISA KitCASP9 适用: Pig Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) ELISA KitCASP9 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) ELISA KitCASP9 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) ELISA KitCASP9 适用: 人 cleaved Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) ELISA KitCASP9 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) ELISA KitCASP9 适用: 小鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) ELISA KitCASP9 适用: 犬 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) ELISA KitSmall Sample CASP9 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
-
-
板类型
- Pre-coated
-
实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
-
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C
-
有效期
- 6 months
-
-
- Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))
-
别名
- Caspase-9
-
基因ID
- 842
-
UniProt
- P55211
-
途径
- MAPK Pathway, RTK signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Endopeptidase Activity
抗原 See all Caspase 9 (CASP9) ELISA试剂盒
-
