CGRP ELISA 试剂盒
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- 抗原 See all CGRP (CALCA) ELISA试剂盒
- CGRP (CALCA) (Calcitonin-Related Polypeptide alpha (CALCA))
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适用
- 人, 大鼠, 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 检测范围
- 0.1 ng/mL - 1000 ng/mL
- 最低检测浓度
- 0.1 ng/mL
- 应用范围
- ELISA
- 原理
- Human/Mouse/Rat CGRP-I EIA Kit optimized for serum, plasma and cell culture media. Competition-based ELISA on a 96-well strip plate.
- 样品类型
- Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This EIA kit is designed to detect human, mouse, and rat Dynorphin
- 灵敏度
- 0.2 ng/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
- Featured
- Discover our best selling CALCA ELISA Kit
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- Discover our top product CALCA ELISA Kit
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Calcitonin-Related Polypeptide alpha (CALCA) ELISA Kit
CALCA 适用: 人 Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Calcitonin-Related Polypeptide alpha (CALCA) ELISA KitCALCA 适用: 大鼠 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Calcitonin-Related Polypeptide alpha (CALCA) ELISA KitCALCA 适用: 小鼠 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Calcitonin-Related Polypeptide alpha (CALCA) ELISA KitCALCA 适用: 小鼠 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Calcitonin-Related Polypeptide alpha (CALCA) ELISA KitCALCA 适用: 大鼠 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Calcitonin-Related Polypeptide alpha (CALCA) ELISA KitCALCA 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Calcitonin-Related Polypeptide alpha (CALCA) ELISA KitCALCA 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Calcitonin-Related Polypeptide alpha (CALCA) ELISA KitCALCA 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Calcitonin-Related Polypeptide alpha (CALCA) ELISA KitCALCA 适用: 人 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Calcitonin-Related Polypeptide alpha (CALCA) ELISA KitCALCA 适用: Pig Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
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- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4 °C.
- Add 100 μL standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 μL prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 μL TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL Stop Solution to each well.
- Read plate at 450 nm immediately.
- 试剂准备
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Recommended Dilution for serum and plasma samplesHuman: sample extraction / Mouse: sample extraction / Rat: sample extraction
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 储存方法
- Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
- 有效期
- 6 months
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- 抗原 See all CGRP (CALCA) ELISA试剂盒
- CGRP (CALCA) (Calcitonin-Related Polypeptide alpha (CALCA))
- 别名
- CGRP-I (CALCA 产品)
- 别名
- CALC1 ELISA Kit, CGRP ELISA Kit, CGRP-I ELISA Kit, CGRP1 ELISA Kit, CT ELISA Kit, KC ELISA Kit, CALC ELISA Kit, CALCA ELISA Kit, CALC3 ELISA Kit, ci-ct ELISA Kit, calca ELISA Kit, CA ELISA Kit, CGRP-1 ELISA Kit, Calc ELISA Kit, Calc1 ELISA Kit, Cgrp ELISA Kit, Ct ELISA Kit, Ctn ELISA Kit, calcitonin ELISA Kit, CAL6 ELISA Kit, Cal1 ELISA Kit, RATCAL6 ELISA Kit, CGRPI ELISA Kit, calc ELISA Kit, cgrp ELISA Kit, zgc:92886 ELISA Kit, calcitonin related polypeptide alpha ELISA Kit, calcitonin related polypeptide beta ELISA Kit, calcitonin-related polypeptide alpha ELISA Kit, calcitonin ELISA Kit, calcitonin/calcitonin-related polypeptide, alpha ELISA Kit, calcitonin-related polypeptide beta ELISA Kit, calcitonin gene-related peptide 2 ELISA Kit, CALCA ELISA Kit, CALCB ELISA Kit, ci-ct ELISA Kit, calca ELISA Kit, Calca ELISA Kit, LOC100008771 ELISA Kit
- 基因ID
- 12310
- UniProt
- Q99JA0
- 途径
- Hormone Activity, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, Feeding Behaviour
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