PPP2R4 ELISA 试剂盒
PPP2R4
适用: 人
Regulatory Subunit 4
Colorimetric
Sandwich ELISA
0.156 ng/mL - 10 ng/mL
Tissue Homogenate
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- 抗原 See all PPP2R4 ELISA试剂盒
- PPP2R4 (Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4))
- 抗原表位
- Regulatory Subunit 4
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 灵敏度
- 0070 ng/mL
- 组件
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - 试剂未包括
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
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Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4) ELISA Kit
PPP2R4 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4) ELISA KitPPP2R4 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 12 months
-
- 抗原 See all PPP2R4 ELISA试剂盒
- PPP2R4 (Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4))
- 别名
- Protein Phosphatase 2A Activator, (PPP2R4) (PPP2R4 产品)
- 别名
- PP2A ELISA Kit, PR53 ELISA Kit, PTPA ELISA Kit, 2610042B21Rik ELISA Kit, C77440 ELISA Kit, N28142 ELISA Kit, PTPRA ELISA Kit, Ppp2r4 ELISA Kit, fc23d07 ELISA Kit, ppp2r4 ELISA Kit, wu:fc23d07 ELISA Kit, zgc:92766 ELISA Kit, pp2a ELISA Kit, ppp2r4.L ELISA Kit, pr53 ELISA Kit, ptpa ELISA Kit, protein phosphatase 2 phosphatase activator ELISA Kit, protein phosphatase 2 protein activator ELISA Kit, protein phosphatase 2 phosphatase activator L homeolog ELISA Kit, PTPA ELISA Kit, Ptpa ELISA Kit, ptpa ELISA Kit, ptpa.L ELISA Kit
- 背景
- Alternative name: PP2A, PR53, PTPA, Phosphotyrosyl Phosphatase Activator, PP2A Phosphatase Activator, PP2A, subunit B', PR53 isoform, Serine/threonine-protein phosphatase 2A activator
- 基因ID
- 5524
- UniProt
- Q15257
- 途径
- PI3K-Akt Signaling, M Phase, Hepatitis C, Toll-Like Receptors Cascades
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