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APPBP2 ELISA 试剂盒

APPBP2 适用: 小鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Tissue Homogenate
产品编号 ABIN5674750
发货至: 中国
  • 抗原 See all APPBP2 ELISA试剂盒
    APPBP2 (Amyloid beta Precursor Protein (Cytoplasmic Tail) Binding Protein 2 (APPBP2))
    适用
    • 1
    • 1
    • 1
    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.312 ng/mL - 20 ng/mL
    最低检测浓度
    0.312 ng/mL
    应用范围
    ELISA
    样品类型
    Tissue Homogenate
    Analytical Method
    Quantitative
    灵敏度
    0115 ng/mL
    组件
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    试剂未包括
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验时间
    1 - 4.5 h
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    12 months
  • 抗原 See all APPBP2 ELISA试剂盒
    APPBP2 (Amyloid beta Precursor Protein (Cytoplasmic Tail) Binding Protein 2 (APPBP2))
    别名
    Amyloid Beta Precursor Protein Binding Protein 2 (APPBP2) (APPBP2 产品)
    别名
    HS.84084 ELISA Kit, PAT1 ELISA Kit, 1300003O07Rik ELISA Kit, AI465480 ELISA Kit, zgc:76971 ELISA Kit, wu:fb78e11 ELISA Kit, wu:fc25c09 ELISA Kit, APPBP2 ELISA Kit, DKFZp459F0530 ELISA Kit, amyloid beta precursor protein binding protein 2 ELISA Kit, amyloid beta precursor protein (cytoplasmic tail) binding protein 2 ELISA Kit, amyloid beta precursor protein (cytoplasmic tail) binding protein 2 S homeolog ELISA Kit, APPBP2 ELISA Kit, Appbp2 ELISA Kit, appbp2 ELISA Kit, appbp2.S ELISA Kit
    背景
    Alternative name: PAT1, Protein Interacting With APP Tail 1,Cytoplasmic Tail, Amyloid protein-binding protein 2, Protein interacting with APP tail 1
    基因ID
    66884
    UniProt
    Q9DAX9
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