COL2A1 ELISA 试剂盒
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- 抗原 See all COL2A1 ELISA试剂盒
- COL2A1 (Collagen, Type II, alpha 1 (COL2A1))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 灵敏度
- 0055 ng/mL
- 组件
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - 试剂未包括
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
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Collagen, Type II, alpha 1 (COL2A1) ELISA Kit
COL2A1 适用: 人 Colorimetric Sandwich ELISA 0.63 ng/mL - 40 ng/mL Cell Culture Supernatant, Plasma, Serum
Collagen, Type II, alpha 1 (COL2A1) ELISA KitCOL2A1 适用: 人 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Collagen, Type II, alpha 1 (COL2A1) ELISA KitCOL2A1 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Collagen, Type II, alpha 1 (COL2A1) ELISA KitCOL2A1 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Collagen, Type II, alpha 1 (COL2A1) ELISA KitCOL2A1 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
Collagen, Type II, alpha 1 (COL2A1) ELISA KitCOL2A1 适用: 大鼠 Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 12 months
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- 抗原 See all COL2A1 ELISA试剂盒
- COL2A1 (Collagen, Type II, alpha 1 (COL2A1))
- 别名
- CollagenType II Alpha 1 (COL2a1) (COL2A1 产品)
- 别名
- ANFH ELISA Kit, AOM ELISA Kit, COL11A3 ELISA Kit, SEDC ELISA Kit, Col2 ELISA Kit, Col2a ELISA Kit, Col2a-1 ELISA Kit, Del1 ELISA Kit, Dmm ELISA Kit, Lpk ELISA Kit, M100856 ELISA Kit, Rgsc856 ELISA Kit, CG2A1A ELISA Kit, COLLII ELISA Kit, col2a1a ELISA Kit, CB11 ELISA Kit, col2a1 ELISA Kit, coll2a1 ELISA Kit, fb38c06 ELISA Kit, fc10c01 ELISA Kit, wu:fb38c06 ELISA Kit, wu:fc10c01 ELISA Kit, collagen type II alpha 1 chain ELISA Kit, collagen, type II, alpha 1 ELISA Kit, collagen, type II, alpha 1 L homeolog ELISA Kit, collagen, type II, alpha 1 S homeolog ELISA Kit, collagen, type II, alpha 1a ELISA Kit, COL2A1 ELISA Kit, Col2a1 ELISA Kit, col2a1 ELISA Kit, col2a1.L ELISA Kit, col2a1.S ELISA Kit, col2a1a ELISA Kit
- 背景
- Alternative name: COL2-A1, COL2A1, SEDC, Chondrocalcin, Collagen Alpha-1(II)Chain, Primary Osteoarthritis,Spondyloepiphyseal Dysplasia,Congenital
- 基因ID
- 12824
- UniProt
- P28481
- 途径
- Sensory Perception of Sound, Growth Factor Binding
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