TSPO ELISA 试剂盒
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- 抗原 See all TSPO ELISA试剂盒
- TSPO (Peripheral-Type Benzodiazepine Receptor (TSPO))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Translocator Protein (TSPO). No significant cross-reactivity or interference between Translocator Protein (TSPO) and analogues was observed.
- 灵敏度
- 0.113 ng/mL
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Peripheral-Type Benzodiazepine Receptor (TSPO) ELISA Kit
TSPO 适用: 小鼠 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Peripheral-Type Benzodiazepine Receptor (TSPO) ELISA KitTSPO 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Peripheral-Type Benzodiazepine Receptor (TSPO) ELISA KitTSPO 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Translocator Protein (TSPO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Translocator Protein (TSPO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Translocator Protein (TSPO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Translocator Protein (TSPO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Translocator Protein (TSPO) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Translocator Protein (TSPO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all TSPO ELISA试剂盒
- TSPO (Peripheral-Type Benzodiazepine Receptor (TSPO))
- 别名
- Translocator Protein (TSPO 产品)
- 别名
- LOC732875 ELISA Kit, LOC100136652 ELISA Kit, LOC100280949 ELISA Kit, BZRP ELISA Kit, TSPO ELISA Kit, Bzrp ELISA Kit, IBP ELISA Kit, PBR ELISA Kit, Tspo1 ELISA Kit, MBR ELISA Kit, PTBZR02 ELISA Kit, Ptbzr ELISA Kit, RATPTBZR02 ELISA Kit, BPBS ELISA Kit, DBI ELISA Kit, PBS ELISA Kit, PKBS ELISA Kit, PTBR ELISA Kit, mDRC ELISA Kit, pk18 ELISA Kit, peripheral-type benzodiazepine receptor ELISA Kit, Peripheral-type benzodiazepine receptor ELISA Kit, putative peripheral-type benzodiazepine receptor ELISA Kit, translocator protein ELISA Kit, translocator protein homolog ELISA Kit, hypothetical protein ELISA Kit, LOC732875 ELISA Kit, MAV_3896 ELISA Kit, xccb100_2086 ELISA Kit, Smp_102510 ELISA Kit, Sros_1911 ELISA Kit, tspo ELISA Kit, LOC100280949 ELISA Kit, PGTG_16395 ELISA Kit, TSPO ELISA Kit, Tspo ELISA Kit
- 背景
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Gene Name: Translocator Protein
Gene Aliases: IBP, PKBS, PBR, pk18, mDRC, MBR, BZRP, DBI, PTBR, Mitochondrial benzodiazepine receptor, Peripheral-Type Benzodiazepine Receptor/Recognition Site
- 基因ID
- 706
- UniProt
- P30536
- 途径
- Cellular Response to Molecule of Bacterial Origin
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