NR5A2 + LRH1 ELISA 试剂盒
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- 抗原 See all NR5A2 + LRH1 (NR5A2) ELISA试剂盒
- NR5A2 + LRH1 (NR5A2) (Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Liver Receptor Homolog 1 (LRH1). No significant cross-reactivity or interference between Liver Receptor Homolog 1 (LRH1) and analogues was observed.
- 灵敏度
- 0.063 ng/mL
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Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2) ELISA Kit
NR5A2 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2) ELISA KitNR5A2 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Liver Receptor Homolog 1 (LRH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Liver Receptor Homolog 1 (LRH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Liver Receptor Homolog 1 (LRH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Liver Receptor Homolog 1 (LRH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Liver Receptor Homolog 1 (LRH1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Liver Receptor Homolog 1 (LRH1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
-
- 抗原 See all NR5A2 + LRH1 (NR5A2) ELISA试剂盒
- NR5A2 + LRH1 (NR5A2) (Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2))
- 别名
- Liver Receptor Homolog 1 (NR5A2 产品)
- 别名
- xff1rashort ELISA Kit, NR5A2 ELISA Kit, b1f ELISA Kit, b1f2 ELISA Kit, cpf ELISA Kit, ff1r ELISA Kit, ftf ELISA Kit, ftz-f1 ELISA Kit, ftz-f1beta ELISA Kit, hb1f ELISA Kit, hb1f-2 ELISA Kit, lrh-1 ELISA Kit, lrh1 ELISA Kit, xff1ra ELISA Kit, B1F ELISA Kit, B1F2 ELISA Kit, CPF ELISA Kit, FTF ELISA Kit, FTZ-F1 ELISA Kit, FTZ-F1beta ELISA Kit, LRH-1 ELISA Kit, LRH1 ELISA Kit, hB1F-2 ELISA Kit, xFF1rB ELISA Kit, AU020803 ELISA Kit, D1Ertd308e ELISA Kit, Ftf ELISA Kit, UF2-H3B ELISA Kit, Lrh-1 ELISA Kit, nuclear receptor subfamily 5 group A member 2 S homeolog ELISA Kit, nuclear receptor subfamily 5 group A member 2 ELISA Kit, nuclear receptor subfamily 5 group A member 2 L homeolog ELISA Kit, nuclear receptor subfamily 5, group A, member 2 ELISA Kit, nr5a2.S ELISA Kit, NR5A2 ELISA Kit, nr5a2 ELISA Kit, nr5a2.L ELISA Kit, Nr5a2 ELISA Kit
- 背景
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Gene Name: Liver Receptor Homolog 1
Gene Aliases: NR5A2, B1F, B1F2, CPF, FTF, FTZ-F1, LRH-1, hB1F, hB1F-2, Hepatocytic transcription factor, Alpha-1-fetoprotein transcription factor, Nuclear Receptor Subfamily 5,Group A,Member 2
- 基因ID
- 60349
- UniProt
- Q9QWM1
- 途径
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway
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