SERPIND1 ELISA 试剂盒
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- 抗原 See all SERPIND1 ELISA试剂盒
- SERPIND1 (serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 7.81 ng/mL - 500 ng/mL
- 最低检测浓度
- 7.81 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Heparin Cofactor II (HCII). No significant cross-reactivity or interference between Heparin Cofactor II (HCII) and analogues was observed.
- 灵敏度
- 3.3 ng/mL
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serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1) ELISA Kit
SERPIND1 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma
serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1) ELISA KitSERPIND1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma
serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1) ELISA KitSERPIND1 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1) ELISA KitSERPIND1 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1) ELISA KitSERPIND1 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1) ELISA KitSERPIND1 适用: 兔 Colorimetric Sandwich ELISA 3.12 U/L - 200 U/L Plasma
serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1) ELISA KitSERPIND1 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma
serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1) ELISA KitSmall Sample SERPIND1 适用: 小鼠 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma
serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1) ELISA KitSERPIND1 适用: 人 Colorimetric Sandwich ELISA 1.23-300 ng/mL Cell Culture Supernatant, Plasma, Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heparin Cofactor II (HCII). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heparin Cofactor II (HCII). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heparin Cofactor II (HCII), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heparin Cofactor II (HCII) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heparin Cofactor II (HCII) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heparin Cofactor II (HCII) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all SERPIND1 ELISA试剂盒
- SERPIND1 (serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1))
- 别名
- Heparin Cofactor II (SERPIND1 产品)
- 别名
- D22S673 ELISA Kit, HC2 ELISA Kit, HCF2 ELISA Kit, HCII ELISA Kit, HLS2 ELISA Kit, LS2 ELISA Kit, THPH10 ELISA Kit, serpind1 ELISA Kit, MGC53936 ELISA Kit, rls2var1 ELISA Kit, AA985900 ELISA Kit, AI303446 ELISA Kit, Hcf2 ELISA Kit, serpin family D member 1 ELISA Kit, serpin peptidase inhibitor, clade D (heparin cofactor), member 1 L homeolog ELISA Kit, serpin peptidase inhibitor, clade D (heparin cofactor), member 1 ELISA Kit, serine (or cysteine) peptidase inhibitor, clade D, member 1 ELISA Kit, SERPIND1 ELISA Kit, serpind1.L ELISA Kit, Serpind1 ELISA Kit, serpind1 ELISA Kit
- 背景
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Gene Name: Heparin Cofactor II
Gene Aliases: SERPIND1, HC2, HCF2, HC II, HLS2, LS2, Serpin D1, Serpin Peptidase Inhibitor Clade D Member 1, Protease inhibitor leuserpin-2
- 基因ID
- 79224
- UniProt
- Q64268
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