SLC40A1 ELISA 试剂盒
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- 抗原 See all SLC40A1 ELISA试剂盒
- SLC40A1 (Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Ferroportin (FPN). No significant cross-reactivity or interference between Ferroportin (FPN) and analogues was observed.
- 灵敏度
- 0.131 ng/mL
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Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1) ELISA Kit
SLC40A1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1) ELISA KitSLC40A1 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1) ELISA KitSLC40A1 适用: 小鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1) ELISA KitSLC40A1 适用: 人 Colorimetric Competition ELISA 246.9 pg/mL - 20000 pg/mL Cell Lysate, Tissue Homogenate
Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1) ELISA KitSLC40A1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1) ELISA KitSLC40A1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1) ELISA KitSLC40A1 适用: Cow Colorimetric Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ferroportin (FPN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ferroportin (FPN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ferroportin (FPN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ferroportin (FPN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ferroportin (FPN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ferroportin (FPN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all SLC40A1 ELISA试剂盒
- SLC40A1 (Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1))
- 别名
- Ferroportin (SLC40A1 产品)
- 别名
- FPN1 ELISA Kit, HFE4 ELISA Kit, IREG1 ELISA Kit, MST079 ELISA Kit, MTP1 ELISA Kit, SLC11A3 ELISA Kit, slc39a1 ELISA Kit, weh ELISA Kit, slc40a1 ELISA Kit, MGC82700 ELISA Kit, SLC40A1 ELISA Kit, Fpn1 ELISA Kit, Slc11a3 ELISA Kit, Slc39a1 ELISA Kit, Dusg ELISA Kit, MTP ELISA Kit, Ol5 ELISA Kit, Pcm ELISA Kit, ARABIDOPSIS THALIANA IRON-REGULATED PROTEIN 1 ELISA Kit, ATIREG1 ELISA Kit, FERROPORTIN 1 ELISA Kit, T19C21.5 ELISA Kit, T19C21_5 ELISA Kit, iron regulated 1 ELISA Kit, ARABIDOPSIS IRON-REGULATED TRANSPORTER 1 ELISA Kit, ATIRT1 ELISA Kit, T16H5.50 ELISA Kit, T16H5_50 ELISA Kit, iron-regulated transporter 1 ELISA Kit, solute carrier family 40 member 1 ELISA Kit, solute carrier family 40 (iron-regulated transporter), member 1 ELISA Kit, solute carrier family 40 (iron-regulated transporter), member 1 S homeolog ELISA Kit, iron regulated 1 ELISA Kit, iron-regulated transporter 1 ELISA Kit, SLC40A1 ELISA Kit, slc40a1 ELISA Kit, slc40a1.S ELISA Kit, Slc40a1 ELISA Kit, IREG1 ELISA Kit, IRT1 ELISA Kit
- 背景
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Gene Name: Ferroportin
Gene Aliases: SLC40A1, FPN1, HFE4, IREG1, MTP1, SLC11A3, Solute Carrier Family 40,Member 1, Iron-Regulated Transporter
- 基因ID
- 30061
- UniProt
- Q9NP59
- 途径
- Transition Metal Ion Homeostasis
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