SLC27A5 ELISA 试剂盒
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- 抗原 See all SLC27A5 ELISA试剂盒
- SLC27A5 (Solute Carrier Family 27 (Fatty Acid Transporter), Member 5 (SLC27A5))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Fatty Acid Transport Protein 5 (FATP5). No significant cross-reactivity or interference between Fatty Acid Transport Protein 5 (FATP5) and analogues was observed.
- 灵敏度
- 0.054 ng/mL
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Solute Carrier Family 27 (Fatty Acid Transporter), Member 5 (SLC27A5) ELISA Kit
SLC27A5 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Solute Carrier Family 27 (Fatty Acid Transporter), Member 5 (SLC27A5) ELISA KitSLC27A5 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Transport Protein 5 (FATP5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fatty Acid Transport Protein 5 (FATP5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fatty Acid Transport Protein 5 (FATP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fatty Acid Transport Protein 5 (FATP5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Transport Protein 5 (FATP5) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Transport Protein 5 (FATP5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all SLC27A5 ELISA试剂盒
- SLC27A5 (Solute Carrier Family 27 (Fatty Acid Transporter), Member 5 (SLC27A5))
- 别名
- Fatty Acid Transport Protein 5 (SLC27A5 产品)
- 别名
- ACSB ELISA Kit, ACSVL6 ELISA Kit, BACS ELISA Kit, BAL ELISA Kit, FACVL3 ELISA Kit, FATP-5 ELISA Kit, FATP5 ELISA Kit, VLACSR ELISA Kit, VLCS-H2 ELISA Kit, VLCSH2 ELISA Kit, Vlacsr ELISA Kit, rBAL-1 ELISA Kit, SLC27A5 ELISA Kit, solute carrier family 27 member 5 ELISA Kit, solute carrier family 27 (fatty acid transporter), member 5 ELISA Kit, bile acyl-CoA synthetase ELISA Kit, SLC27A5 ELISA Kit, Slc27a5 ELISA Kit, LOC608675 ELISA Kit, LOC100450572 ELISA Kit
- 背景
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Gene Name: Fatty Acid Transport Protein 5
Gene Aliases: SLC27A5, ACSB, ACSVL6, FACVL3, VLACSR, BACS, VLCSH2, Fatty Acid Coenzyme A Ligase Very Long Chain 3, Solute Carrier Family 27 Member 5, Bile Acyl CoA Synthetase
- 基因ID
- 26459
- UniProt
- Q4LDG0
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