ELAVL1 ELISA 试剂盒
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- 抗原 See all ELAVL1 ELISA试剂盒
- ELAVL1 (ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-Like 1 (Hu Antigen R) (ELAVL1))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of ELAV Like Protein 1 (ELAVL1). No significant cross-reactivity or interference between ELAV Like Protein 1 (ELAVL1) and analogues was observed.
- 灵敏度
- 0.066 ng/mL
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ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-Like 1 (Hu Antigen R) (ELAVL1) ELISA Kit
ELAVL1 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-Like 1 (Hu Antigen R) (ELAVL1) ELISA KitELAVL1 适用: 人 Colorimetric Sandwich ELISA 25 pg/mL - 1600 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-Like 1 (Hu Antigen R) (ELAVL1) ELISA KitELAVL1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-Like 1 (Hu Antigen R) (ELAVL1) ELISA KitELAVL1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ELAV Like Protein 1 (ELAVL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ELAV Like Protein 1 (ELAVL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ELAV Like Protein 1 (ELAVL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ELAV Like Protein 1 (ELAVL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ELAV Like Protein 1 (ELAVL1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ELAV Like Protein 1 (ELAVL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all ELAVL1 ELISA试剂盒
- ELAVL1 (ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-Like 1 (Hu Antigen R) (ELAVL1))
- 别名
- ELAV Like Protein 1 (ELAVL1 产品)
- 别名
- ELAV1 ELISA Kit, HUR ELISA Kit, Hua ELISA Kit, MelG ELISA Kit, HuR ELISA Kit, 2410055N02Rik ELISA Kit, W91709 ELISA Kit, elra ELISA Kit, fa25f08 ELISA Kit, fb59a02 ELISA Kit, fc60g02 ELISA Kit, hua ELISA Kit, wu:fa25f08 ELISA Kit, wu:fb59a02 ELISA Kit, wu:fc60g02 ELISA Kit, zHuA ELISA Kit, Elavl1 ELISA Kit, Xel-1 ELISA Kit, elav1 ELISA Kit, elrA ELISA Kit, hur ELISA Kit, melg ELISA Kit, p36 ELISA Kit, ElrA ELISA Kit, elavl1 ELISA Kit, elavl1-a ELISA Kit, elavl1-b ELISA Kit, ELAV like RNA binding protein 1 ELISA Kit, ELAV-like protein 1 ELISA Kit, ELAV (embryonic lethal, abnormal vision)-like 1 (Hu antigen R) ELISA Kit, ELAV like RNA binding protein 1a ELISA Kit, ELAV like RNA binding protein 1 L homeolog ELISA Kit, ELAV like RNA binding protein 1 S homeolog ELISA Kit, ELAVL1 ELISA Kit, Elavl1 ELISA Kit, elav1 ELISA Kit, elavl1a ELISA Kit, elavl1.L ELISA Kit, elavl1.S ELISA Kit
- 背景
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Gene Name: ELAV Like Protein 1
Gene Aliases: ELAV1, HUR, Hua, MelG, Hu Antigen R, Embryonic Lethal,Abnormal Vision Like 1
- 途径
- AMPK Signaling
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