CYP1A1 ELISA 试剂盒
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- 抗原 See all CYP1A1 ELISA试剂盒
- CYP1A1 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Cytochrome P450 1A1 (CYP1A1). No significant cross-reactivity or interference between Cytochrome P450 1A1 (CYP1A1) and analogues was observed.
- 灵敏度
- 0.129 ng/mL
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Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1) ELISA Kit
CYP1A1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1) ELISA KitCYP1A1 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1) ELISA KitCYP1A1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1) ELISA KitCYP1A1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1) ELISA KitCYP1A1 适用: 猴 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1) ELISA KitCYP1A1 适用: 鱼 Colorimetric Sandwich ELISA 75-1200 pg/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 1A1 (CYP1A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 1A1 (CYP1A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 1A1 (CYP1A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 1A1 (CYP1A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 1A1 (CYP1A1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 1A1 (CYP1A1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all CYP1A1 ELISA试剂盒
- CYP1A1 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1))
- 别名
- Cytochrome P450 1A1 (CYP1A1 产品)
- 别名
- AHH ELISA Kit, AHRR ELISA Kit, CP11 ELISA Kit, CYP1 ELISA Kit, P1-450 ELISA Kit, P450-C ELISA Kit, P450DX ELISA Kit, Cyp1a2 ELISA Kit, P450-1 ELISA Kit, cyp1a1 ELISA Kit, CYP1A1 ELISA Kit, CYPIA1 ELISA Kit, Cyp45c ELISA Kit, Cypc45c ELISA Kit, P-450MC ELISA Kit, wu:fb63b04 ELISA Kit, zfCYP1A ELISA Kit, zgc:109747 ELISA Kit, ahh ELISA Kit, ahrr ELISA Kit, cp11 ELISA Kit, cyp1 ELISA Kit, cyp1a ELISA Kit, p1-450 ELISA Kit, p450-c ELISA Kit, p450dx ELISA Kit, cytochrome P450 family 1 subfamily A member 1 ELISA Kit, cytochrome P450 1A1 ELISA Kit, cytochrome P450, family 1, subfamily a, polypeptide 1 ELISA Kit, cytochrome P450, family 1, subfamily A, polypeptide 1 ELISA Kit, cytochrome P450 family 1 subfamily D polypeptide 1 ELISA Kit, cytochrome P4501A1 ELISA Kit, polycyclic hydrocarbon-inducible cytochrome P450c ELISA Kit, cytochrome P450, family 1, subfamily A ELISA Kit, cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1 ELISA Kit, cytochrome P450 family 1 subfamily A member 1 L homeolog ELISA Kit, CYP1A1 ELISA Kit, CpipJ_CPIJ010542 ELISA Kit, Cyp1a1 ELISA Kit, cyp1d1 ELISA Kit, LOC100328613 ELISA Kit, cyp1a ELISA Kit, LOC102129994 ELISA Kit, cyp1a1.L ELISA Kit
- 背景
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Gene Name: Cytochrome P450 1A1
Gene Aliases: CYP1, AHH, AHRR, CP11, CYP1, P1-450, P450-C, P450DX, Cytochrome P450,Subfamily I(Aromatic Compound-Inducible),Polypeptide 1
- 基因ID
- 24296
- UniProt
- P00185
- 途径
- Steroid Hormone Biosynthesis, Regulation of Lipid Metabolism by PPARalpha
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