CDC42 ELISA 试剂盒
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- 抗原 See all CDC42 ELISA试剂盒
- CDC42 (Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Cell Division Cycle Protein 42 (CDC42). No significant cross-reactivity or interference between Cell Division Cycle Protein 42 (CDC42) and analogues was observed.
- 灵敏度
- 0.064 ng/mL
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Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42) ELISA Kit
CDC42 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42) ELISA KitCDC42 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cell Division Cycle Protein 42 (CDC42). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cell Division Cycle Protein 42 (CDC42). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cell Division Cycle Protein 42 (CDC42), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cell Division Cycle Protein 42 (CDC42) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cell Division Cycle Protein 42 (CDC42) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cell Division Cycle Protein 42 (CDC42) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all CDC42 ELISA试剂盒
- CDC42 (Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42))
- 别名
- Cell Division Cycle Protein 42 (CDC42 产品)
- 别名
- CDC42Hs ELISA Kit, G25K ELISA Kit, AI747189 ELISA Kit, AU018915 ELISA Kit, cdc42a ELISA Kit, wu:fb07d08 ELISA Kit, wu:fj62g06 ELISA Kit, zgc:63918 ELISA Kit, Xcdc42 ELISA Kit, cdc42hs ELISA Kit, g25k ELISA Kit, CDC42 ELISA Kit, CG12530 ELISA Kit, Cdc42Dm ELISA Kit, D-CDC42 ELISA Kit, D-Cdc42 ELISA Kit, D-cdc42 ELISA Kit, DCDC42 ELISA Kit, DCdc42 ELISA Kit, Dcdc42 ELISA Kit, Dm Cdc42 ELISA Kit, DmCDC42 ELISA Kit, Dmcdc42 ELISA Kit, Dmel\\CG12530 ELISA Kit, cdc42 ELISA Kit, cell division cycle 42 ELISA Kit, Cell division control protein 42 homolog ELISA Kit, cell division control protein 42 homolog ELISA Kit, cell division cycle 42 L homeolog ELISA Kit, CG12530 gene product from transcript CG12530-RC ELISA Kit, Rho family GTPase ELISA Kit, CDC42 ELISA Kit, Cdc42 ELISA Kit, cdc42 ELISA Kit, LOC100636735 ELISA Kit, LOC100637699 ELISA Kit, LOC100638834 ELISA Kit, cdc42.L ELISA Kit
- 背景
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Gene Name: Cell Division Cycle Protein 42
Gene Aliases: CDC42Hs, G25K, G25K GTP-binding protein
- 基因ID
- 998
- UniProt
- P60953
- 途径
- MAPK Pathway, Microtubule Dynamics, RTK signaling, WNT signaling, TCR Signaling, EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Maintenance of Protein Location, Skeletal Muscle Fiber Development, Signaling Events mediated by VEGFR1 and VEGFR2, EGFR Downregulation, VEGF Signaling
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