Calmodulin 1 ELISA 试剂盒
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- 抗原 See all Calmodulin 1 (Calm1) ELISA试剂盒
- Calmodulin 1 (Calm1)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Calmodulin (CAM). No significant cross-reactivity or interference between Calmodulin (CAM) and analogues was observed.
- 灵敏度
- 0.072 ng/mL
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Calmodulin 1 (Calm1) ELISA Kit
Calm1 适用: 人 Colorimetric Sandwich ELISA 93.75 pg/mL - 6000 pg/mL Cell Culture Supernatant, Plasma, Serum
Calmodulin 1 (Calm1) ELISA KitCalm1 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Calmodulin 1 (Calm1) ELISA KitCalm1 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Calmodulin 1 (Calm1) ELISA KitCalm1 适用: 人 Colorimetric Sandwich ELISA 93.75 pg/mL - 6000 pg/mL Plasma, Serum, Tissue Homogenate
Calmodulin 1 (Calm1) ELISA KitCalm1 适用: 兔 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calmodulin (CAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calmodulin (CAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calmodulin (CAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calmodulin (CAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calmodulin (CAM) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calmodulin (CAM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all Calmodulin 1 (Calm1) ELISA试剂盒
- Calmodulin 1 (Calm1)
- 别名
- Calmodulin (Calm1 产品)
- 别名
- AI256814 ELISA Kit, AI327027 ELISA Kit, AI461935 ELISA Kit, AL024000 ELISA Kit, CaM ELISA Kit, Calm ELISA Kit, CaMI ELISA Kit, CALML2 ELISA Kit, CAMI ELISA Kit, CPVT4 ELISA Kit, DD132 ELISA Kit, LQT14 ELISA Kit, PHKD ELISA Kit, caM ELISA Kit, calm1 ELISA Kit, calm2-a ELISA Kit, calm2-b ELISA Kit, calm2a ELISA Kit, calml2 ELISA Kit, cami ELISA Kit, dd132 ELISA Kit, 3909 ELISA Kit, CAM ELISA Kit, CG8472 ELISA Kit, Cal ELISA Kit, Cal49A ELISA Kit, CalA ELISA Kit, Cam1 ELISA Kit, D-CaM ELISA Kit, DmCaM ELISA Kit, DmCalm ELISA Kit, Dmel\CG8472 ELISA Kit, anon-EST:Posey59 ELISA Kit, cam ELISA Kit, dCaM ELISA Kit, l(2)03909 ELISA Kit, l(2)3909 ELISA Kit, lincRNA.S2634 ELISA Kit, calmodulin 1 ELISA Kit, calmodulin 2 ELISA Kit, calmodulin 1 (phosphorylase kinase, delta) L homeolog ELISA Kit, Calmodulin ELISA Kit, Calm1 ELISA Kit, Calm2 ELISA Kit, CALM1 ELISA Kit, calm1.L ELISA Kit, Cam ELISA Kit
- 背景
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Gene Name: Calmodulin
Gene Aliases: CALcium MODULated ProteIN
- 基因ID
- 801
- UniProt
- P62158
- 途径
- cAMP Metabolic Process, Myometrial Relaxation and Contraction, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Phototransduction, BCR Signaling
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